Publikationsdatenbank

Generation of full-thickness skin equivalents using hair follicle-derived primary human keratinocytes and fibroblasts (2018)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Löwa, Anna

Vogt, Annika

Kaessmeyer, Sabine (WE 1)

Hedtrich, Sarah

Quelle

Journal of tissue engineering and regenerative medicine

Bandzählung: 12

Heftzählung: 4

Seiten: e2134 – e2146

ISSN: 1932-7005

Sprache

Englisch

Verweise

DOI: 10.1002/term.2646

Pubmed: 29377584

Kontakt

Institut für Veterinär-Anatomie

Koserstr. 20
14195 Berlin
+49 30 838 75784
anatomie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Skin equivalents are increasingly used as human-based test systems for basic and preclinical research. Most of the established skin equivalents are composed of primary keratinocytes and fibroblasts, isolated either from excised human skin or juvenile foreskin following circumcisions. Although the potential of hair follicle-derived cells for the generation of skin equivalents has been shown, this approach normally requires microdissections from the scalp for which there is limited subject compliance or ethical approval. In the present study, we report a novel method to isolate and cultivate keratinocytes and fibroblasts from plucked hair follicles that were then used to generate skin equivalents. The procedure is non-invasive, inflicts little-pain, and may allow easy access to patient-derived cells without taking punch biopsies. Overall, minor differences in morphology, ultrastructure, expression of important structural proteins, or barrier function were observed between skin equivalents generated from hair follicle-derived or interfollicular keratinocytes and fibroblasts. Interestingly, improved basal lamina formation was seen in the hair follicle-derived skin equivalents. The presented method here allows easy and non-invasive access to keratinocytes and fibroblasts from plucked hair follicles that may be useful particularly for the generation of skin disease equivalents.