Publikationsdatenbank

In vitro Feeding Methods for Hematophagous Arthropods and Their Application in Drug Discovery (2018)

Art

Buchbeitrag

Autoren

Nijhof, Ard Menzo (WE 13)

Tyson, Katharina R

Quelle

Ectoparasites: Drug Discovery Against Moving Targets — Meng, C.Q. & Sluder, A.E. (Hrsg.)

Weinheim, Germany: Wiley‐VCH Verlag GmbH & Co., 2018. Drug Discovery in Infectious Diseases ; 8 — S. 187–204

ISBN: 978-3-527-34168-9

Sprache

Englisch

Verweise

URL (Volltext): https://onlinelibrary.wiley.com/doi/book/10.1002/9783527802883

DOI: 10.1002/9783527802883.ch9

Kontakt

Institut für Parasitologie und Tropenveterinärmedizin

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 62310
parasitologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

In ectoparasiticide research, the use of artificial feeding systems (AFSs) to evaluate the in vitro efficacy of test compounds against hematophagous arthropods offers many potential advantages: only small amounts of test compounds are usually required, compound effects can be tested in a controlled laboratory setting with limited variables, and their use leads to a reduction in experimental animal use. However, initial assay development can be challenging and not all hematophagous arthropods can be maintained under laboratory conditions using AFSs. In this chapter, general requirements for the main components of each AFS, including the membrane, blood meal, and temperature control system are discussed and an overview of currently established in vitro feeding methods for major hematophagous arthropod groups is presented. AFSs have found successful application in drug discovery projects targeting fleas, which may serve as an example for the further development of improved and more consistent assays employing AFSs.