Publikationsdatenbank

Role of ascorbic acid, serum lipids and acetic acid during transdifferentiation of bovine pre-adipocytes to adipocytes (2018)

Art

Poster

Autoren

Jurek, S (WE 2)

Sandhu, M A

Aschenbach, J R (WE 2)

Kolisek, M

Sponder, G (WE 2)

Trappe, S (WE 2)

Kongress

23. Tagung der DVG-Fachgruppe Physiologie und Biochemie der Deutschen Veterinärmedizinischen Gesellschaft

Wien, 21. – 23.02.2018

Quelle

23. Tagung der Fachgruppe Physiologie und Biochemie der Deutschen Veterinärmedizinischen Gesellschaft : PROGRAMM & ABSTRACTS — Veterinärmedizinische Universität Wien (Hrsg.)

— S. 81

Sprache

Englisch

Verweise

URL (Volltext): http://www.vetmeduni.ac.at/fileadmin/v/DVG-Tagung-2018/160218_Abstract_Program_Guide_sortiert_final.pdf

Kontakt

Institut für Veterinär-Physiologie

Oertzenweg 19 b
14163 Berlin
+49 30 838 62600
physiologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

The differentiation of pre-adipocytes to adipocytes is depending on many factors. The aim of the present study was to investigate the effects of ascorbic acid (AA), bovine serum lipids (BSL) and acetic acid on the adipogenic potential of bovine pre-adipocytes during differentiation to adipocytes.
Methods: Bovine pre-adipocytes were grown from explant cultures. After 2 d of induction, pre-adipocytes were incubated in media either containing or not containing AA (40 μl/ml), BSL (10 μl/ml) and fetal bovine serum (FBS, 10%) in a three-factorial design. In a second experiment, the effects of glucose concentration (10 or 25 mM) in the copresence of various acetic acid concentrations (0, 10 or 20 mM) were tested. Culture plates had different coatings, i.e., collagen A, gelatin A or poly-L-lysine in comparison to no coating. Adipogenic effects were assessed based on Nile-red staining of cellular non-polar lipids and by quantitative RT-PCR of stem cell and adipocyte markers.
Results: The accumulation of lipid droplets was enhanced (P < 0.001) in treatments containing BSL but no FBS compared to all other treatments. DAPI staining, as an indirect measure of cell number, was more intense (P < 0.001) in media containing FBS. The mRNA expression of FabP4 increased (P < 0.001) in FBS-free media supplemented with BSL, and was additionally promoted by AA. The mRNA expression of CD73, CD90 and CD105 was lowest (P < 0.001) in treatments devoid of FBS, except for CD90 where AA prevented such decrease. In the second experiment, the concentration of glucose had no effect on the accumulation of non-polar lipids. The DAPI signal decreased (P < 0.01) after 14 d as compared to 7 d; whereas, the accumulation of non-polar lipids was higher (P < 0.01) after 14 d with no interaction between these factors and the factors coating and acetic acid concentration. Regarding the latter, a non-significant linear increase in non-polar lipid accumulation was observed with increasing acetic acid concentration. The DAPI signal was lower (P < 0.001) in uncoated and collagen A coated plates compared with poly-L-lysine and gelatin A coated plates.
Conclusions: Differentiation of bovine pre-adipocytes is promoted by BSL and acetic acid but inhibited by FBS. Differentiation includes an induction of FabP4 expression, a decrease in CD73, CD105 and, in part, CD90 expression, and is affected by AA. Poly-L-lysine or gelatin A coating improves the retention of differentiating adipocytes on culture plates.