Publikationsdatenbank

Campylobacter jejuni modulates the intestinal mucosal barrier function with consequences on bacterial translocation in chickens (2018)

Art

Vortrag

Autoren

Awad, W

Hess, C

Aschenbach, J R (WE 2)

Dublecz, K

Hess, M

Kongress

23. Tagung der DVG-Fachgruppe Physiologie und Biochemie der Deutschen Veterinärmedizinischen Gesellschaft

Wien, 21. – 23.02.2018

Quelle

23. Tagung der Fachgruppe Physiologie und Biochemie der Deutschen Veterinärmedizinischen Gesellschaft : PROGRAMM & ABSTRACTS — Veterinärmedizinische Universität Wien (Hrsg.)

— S. V26

Sprache

Englisch

Verweise

URL (Volltext): http://www.vetmeduni.ac.at/fileadmin/v/DVG-Tagung-2018/160218_Abstract_Program_Guide_sortiert_final.pdf

Kontakt

Institut für Veterinär-Physiologie

Oertzenweg 19 b
14163 Berlin
+49 30 838 62600
physiologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

The intestinal epithelial barrier serves as the first line of defence between the host and thevluminal environment. There is considerable evidence that a dysfunction of the intestinal barrier is an important factor contributing to the pathogenicity of some enteric bacteria (e.g.
Campylobacter jejuni). For a long time Campylobacter was only considered as a commensal in avian hosts restricted to the ceca, without any pathogenic features. However, recent studies challenge this belief. In experimental studies it was noticed for some Campylobacter isolates that the colonization of the gut has a negative impact on birds’ health and well-being. For example, C. jejuni disrupts the intestinal barrier which results in a higher intestinal permeability. This effect promotes the translocation of bacteria to internal organs, an effect not solely restricted to Campylobacter itself. Accordingly, not only C. jejuni can cross the intestinal epithelial barrier, but the translocation of other enteric microorganisms such as E. coli to extra-intestinal organs is facilitated as well. Changes in the intestinal barrier function characterized by increased intestinal permeability, downregulation of certain nutrient transporters and an induction of mucous production display severe consequences on nutrient uptake. Consistent with the latter facts, it was found that glucose uptake and amino acid availability were reduced following C. jejuni infection. Furthermore, it was revealed by twophoton microscopy that intracellular calcium [Ca2+]i was highly up-regulated in the intestinal mucosa of infected birds, indicating that the modulation of [Ca2+]i by Campylobacter might be involved in facilitating the necessary cytoskeletal rearrangements that occur during the bacterial invasion of epithelial cells. Moreover, C. jejuni colonization could be associated with an alteration of the gut microbiota as infected birds had a significantly lower abundance of E. coli at different gut sites. On the contrary, the level of Clostridium spp. was higher in infected birds compared with control birds, demonstrating that the infection of chickens with C. jejuni was associated with significant changes in the composition of the intestinal ecosystem. Altogether, these findings indicate a somewhat intense interaction between C. jejuni and its avian host. In fact, the Campylobacter-host interaction in the gut is characterized by a loss of integrity of the mucosal barrier.