Publikationsdatenbank

Influence of dietary menthol-based bioactive lipid compounds on conductance and uptakes of glucose and methionine in the rumen and intestine of sheep (2018)

Art

Vortrag

Autoren

Patra, A K

Geiger, S (WE 2)

Braun, H S (WE 2)

Aschenbach, J R (WE 2)

Kongress

23. Tagung der DVG-Fachgruppe Physiologie und Biochemie der Deutschen Veterinärmedizinischen Gesellschaft

Wien, 21. – 23.02.2018

Quelle

23. Tagung der Fachgruppe Physiologie und Biochemie der Deutschen Veterinärmedizinischen Gesellschaft : PROGRAMM & ABSTRACTS — Veterinärmedizinische Universität Wien (Hrsg.)

Wien, 2018 — S. V25

Sprache

Englisch

Verweise

URL (Volltext): http://www.vetmeduni.ac.at/fileadmin/v/DVG-Tagung-2018/160218_Abstract_Program_Guide_sortiert_final.pdf

Kontakt

Institut für Veterinär-Physiologie

Oertzenweg 19 b
14163 Berlin
+49 30 838 62600
physiologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

The present study was conducted to investigate if dietary plant bioactive lipid compounds (PBLC) with menthol as a lead substance could influence the uptakes of glucose and methionine (Met) and electrophysiological properties of the rumen and intestine of sheep.

Methods: Growing Suffolk sheep (n = 24) were equally distributed to three diet supplemented with no PBLC (diet 1), 80 mg/d of PBLC (OAX17, PerformaNat GmbH; diet 2) and 160 mg/d of PBLC (diet 3) in a randomised block design based on initial body weight and sex. After 28 d of supplementation, epithelia from the ventral rumen and mid-jejunum were collected for ex vivo measurements of conductance (Gt) and glucose and Met uptake using Ussing chambers. Uptakes of glucose (200 μM) and Met (50 μM) were measured in the presence or absence of Na on the mucosal side. Data were analysed using mixed model procedures of SAS.

Results: In the rumen, baseline Gt increased linearly with increasing doses of PBLC (3.21, 3.62 and 4.37 mS/cm2 for diet 1, 2 and 3, respectively; linear P = 0.023 and SE = 0.34). Glucose uptakes in the presence (mean = 254 pmol/cm2/min; linear P = 0.55; SE = 43.3) or absence (mean = 217 pmol/cm2/min; linear P = 0.11; SE = 21.4) of Na+ were not affected by the PBLC. Met uptakes were also not affected by the PBLC in the presence of Na+ (mean = 62.7 pmol/cm2/min; linear P = 0.40; SE = 12.2), but showed a linear increase due to increasing doses of PBLC (50.3, 45.7 and 67.9 pmol/cm2/min for diet 1, 2 and 3, respectively; linear P = 0.05; SE = 5.92) in the absence of Na+. The absence of Na+ tended to decrease ruminal glucose (P = 0.10) but not Met uptake (P = 0.24). In the jejunum, Gt and uptakes of glucose and Met in the presence or absence of Na+ were not influenced (P > 0.10) by the PBLC. Intestinal uptakes of glucose (1283 vs. 827 pmol/cm2/min; SE = 82.4; P < 0.001) and Met (727 versus 421 pmol/cm2/min; SE = 46.2; P < 0.001) were different in the presence vs. absence of Na+.

Conclusions: Supplementing PBLC exert an enhancing effect on the ion permeability of the ruminal epithelium and no effect on glucose uptake. The Na+-independent absorption of Met in the rumen appears to be increased by PBLC; however, ruminal Met uptake is very small and not clearly Na+-dependent. No effects of PBLC were evident on the investigated variables in the intestine.
This study was conducted with partial funding by PerformaNat GmbH, Germany. AKP acknowledges the Fellowship of the Alexander von Humboldt Foundation.