Publikationsdatenbank

Dietary methionine source influences the expression profile of methionine transport systems in the gastrointestinal tract of growing pigs (2018)

Art

Vortrag

Autoren

Mastrototaro, L (WE 2)

Romanet, S (WE 2)

Pieper, R (WE 4)

Zentek, J (WE 4)

Saremi, B

HToo, J K

Aschenbach, J R (WE 2)

Kongress

23. Tagung der DVG-Fachgruppe Physiologie und Biochemie der Deutschen Veterinärmedizinischen Gesellschaft

Wien, 21. – 23.02.2018

Quelle

23. Tagung der Fachgruppe Physiologie und Biochemie der Deutschen Veterinärmedizinischen Gesellschaft : PROGRAMM & ABSTRACTS — Veterinärmedizinische Universität Wien (Hrsg.)

— S. V23

Sprache

Englisch

Verweise

URL (Volltext): http://www.vetmeduni.ac.at/fileadmin/v/DVG-Tagung-2018/160218_Abstract_Program_Guide_sortiert_final.pdf

Kontakt

Institut für Veterinär-Physiologie

Oertzenweg 19 b
14163 Berlin
+49 30 838 62600
physiologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Introduction: Methionine (Met) is an essential amino acid (AA) that often requires dietary supplementation in farm animals for optimal health and performance. A previous study showed that dietary DL-Met supplementation increased Met absorption in the porcine small intestine. The present project explored the distribution of various Met transporters along the gastrointestinal tract (GIT) of growing pigs supplemented with either L-Met, DL-Met or the Met precursor DL-2-hydroxy-4-(methylthio)butanoate (DL-HMTBA).

Methods: A total of 27 piglets (10 weeks old) were randomly allocated to 3 feeding groups receiving diets supplemented with either 0.21% DL-Met (diet 1), 0.21% L-Met (diet 2) or 0.31% DL-HMTBA (diet 3) to meet Met+Cys requirement. Expression analysis of selected Met transport proteins was conducted in 11 gastrointestinal (GI) regions using quantitative real-time PCR and, in part, by Western blot.
Results: Relative gene expression was different among the GI sections for all genes evaluated (P < 0.001); however, ASCT2, SNAT2 and IMINO showed only moderately heterogeneous expression across GI segments. The B0AT1, b0,+, y+LAT1 and LAT4 showed high expression in the small intestine with B0AT1, y+LAT1, LAT2 and LAT4 dominating in the mid-jejunum, and b0,+ dominating in the proximal jejunum. In the small intestinal segments, the mRNA expression of ASCT2 was increased by diet 1 and the mRNA expression of LAT2 was increased by diet 3. The protein levels of B0AT1 in the small intestine were greatest in the ileum (P < 0.001) wherein they were increased by diet 1 (P < 0.001). LAT4 protein was increased in small intestinal and extraintestinal regions (oral mucosa, stomach) by diet 3.

Conclusion: A diet containing DL-Met upregulates the Na+-dependent apical systems ASCT2 at the mRNA level and B0AT1 at the protein level in the small intestine. A diet containing DL-HMTBA is linked to higher expression of the basolateral systems LAT4 and LAT2 in the small intestine which can help to provide enough Met to the epithelial cells via blood because these cells do not receive free Met from diet. The higher expression of ASCT2 and/or B0AT1 could provide an explanation for the higher Na+-dependent Met transport in pigs fed a DL-Met-containing diet.