Publikationsdatenbank

TSLP is a direct trigger for T cell migration in filaggrin-deficient skin equivalents (2017)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Wallmeyer, Leonie (WE 12)

Dietert, Kristina (WE 12)

Sochorová, Michaela (WE 12)

Gruber, Achim D (WE 12)

Kleuser, Burkhard (WE 12)

Vávrová, Kateřina (WE 12)

Hedtrich, Sarah (WE 12)

Quelle

Scientific reports

Bandzählung: 7

Heftzählung: 1

Seiten: 774

ISSN: 2045-2322

Sprache

Englisch

Verweise

DOI: 10.1038/s41598-017-00670-2

Pubmed: 28377574

Kontakt

Institut für Tierpathologie

Robert-von-Ostertag-Str. 15
14163 Berlin
+49 30 838 62450
pathologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Mutations in the gene encoding for filaggrin (FLG) are major predisposing factors for atopic dermatitis (AD). Besides genetic predisposition, immunological dysregulations considerably contribute to its pathophysiology. For example, thymic stromal lymphopoietin (TSLP) is highly expressed in lesional atopic skin and significantly contributes to the pathogenesis of AD by activating dendritic cells that then initiate downstream effects on, for example, T cells. However, little is known about the direct interplay between TSLP, filaggrin-deficient skin and other immune cells such as T lymphocytes. In the present study, FLG knockdown skin equivalents, characterised by intrinsically high TSLP levels, were exposed to activated CD4+ T cells. T cell exposure resulted in an inflammatory phenotype of the skin equivalents. Furthermore, a distinct shift from a Th1/Th17 to a Th2/Th22 profile was observed following exposure of T cells to filaggrin-deficient skin equivalents. Interestingly, TSLP directly stimulated T cell migration exclusively in filaggrin-deficient skin equivalents even in the absence of dendritic cells, indicating a hitherto unknown role of TSLP in the pathogenesis of AD.