Publikationsdatenbank

Comparison of Skin Penetration, Pathological and Clinical Effects of Novel Drug Transporters – Thermoresponsive Nanogels and Core Multishell Nanocarriers – in a Murine Model of Atopic Dermatitis (2018)

Art

Poster

Autoren

Pischon, Hannah (WE 12)

Radbruch, Moritz (WE 12)

Du, Fang

Guilbudagian, Michael

Unbehauen, Michael

Haag, Rainer

Kleuser, Burkhard

Gruber, Achim Dieter (WE 12)

Mundhenk, Lars (WE 12)

Calderón, Marcelo

Kongress

Cutting Edge Pathology 2017 (15th European Congress of Toxicologic Pathology 35th meeting of the ESVP, 28th meeting of the ECVP)

Lyon, 30.08. – 02.09.2017

Quelle

Journal of comparative pathology

Bandzählung: 158

Heftzählung: 1

Seiten: 110

ISSN: 0021-9975

Sprache

Englisch

Verweise

DOI: 10.1016/j.jcpa.2017.10.044

Kontakt

Institut für Tierpathologie

Robert-von-Ostertag-Str. 15
14163 Berlin
+49 30 838 62450
pathologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Introduction: Core multishell nanocarriers (CMS) and thermoresponsive dPG_pNIPAM based nanogels (NG) are promising novel concepts for topical delivery of drugs to treat inflammatory skin diseases. These systems are designed to enhance dermal drug uptake; however, the carriers themselves might also penetrate inflamed skin, possibly eliciting biological effects themselves. Here, we evaluated their penetration, histopathological and clinical effects after topical application in a murine dermatitis model.
Materials and Methods: Atopic dermatitis was induced chemically in SKH-1 mice. Fluorescently labelled, unloaded CMS, NG or solvents were applied topically to the inflamed skin. Clinical parameters including transepidermal water loss, skin hydration, erythema, ear thickness and a clinical score were assessed. Fluorescence microscopy was performed to localize the carriers. Epidermal thickness and degree of inflammation were characterized histologically.
Results: Both CMS and NG were localized in the stratum corneum only, with no penetration into the viable skin layers or beyond. No adverse clinical or histological effects of the carriers were observed in any of the parameters evaluated.
Conclusions: Unloaded CMS and NG appear not to aggravate atopic dermatitis in mice, unlike other nanoparticles. They may therefore serve as the basis for novel promising and biocompatible concepts for drug delivery in atopic dermatitis.