Fachbereich Veterinärmedizin



    Evaluation of the effect of tongue ties on pharyngeal and laryngeal diameters and to prevent the occurrence of DDSP in racehorses (2017)

    Barton, Ann-Kristin (WE 17)
    Troppenz, A.
    Teschner, D.
    Lindenberg, I.
    Merle, R.
    Gehlen, Heidrun
    10th Annual European College of Equine Internal Medicine Congress
    Budapest, 02. – 04.11.2017
    Journal of veterinary internal medicine; 32(2) — S. 874
    ISSN: 0891-6640
    URL (Volltext): https://www.researchgate.net/profile/Tarek_Shety/publication/325348408_Misbalance_of_Elastinolytic_Activity_in_Equine_Asthma_Positively_Influenced_by_CPG-ODN_Inhalation_Therapy/links/5b0738d0aca2725783e04e24/Misbalance-of-Elastinolytic-Activity-in-Equine-Asthma-Positively-Influenced-by-CPG-ODN-Inhalation-Therapy.pdf?origin=publication_detail
    Klinik für Pferde, allgemeine Chirurgie und Radiologie

    Oertzenweg 19 b
    14163 Berlin
    +49 30 838 62299

    Abstract / Zusammenfassung

    Objectives: Inhalation of immunostimulatory bacterial DNA segments (cytosine-phosphate-guanosine-oligodeoxynucleotides, CpGODN)
    has been shown to normalize clinical and cytologic parameters in severe equine asthma (recurrent airway obstruction, RAO). We
    hypothesized that CpG-ODN inhalation reduces the misbalance of elastinolytic activity in horses affected by RAO.
    Methods: 20 horses diagnosed as RAO by clinical examinations using a scoring system were included. All horses were treated with
    inhalative CpG-ODN for 14 days in 2-day intervals. Matrixmetalloproteinase (MMP-2/-9) and tissue inhibitors of metalloproteinases
    (TIMP-1/-2) concentrations were measured in tracheal aspirates using equine ELISA kits (USCN Life Science Inc.) before, immediately
    and 6 weeks after CpG-ODN inhalation.
    Results: MMP and TIMP concentrations correlated with the results of clinical scoring in all stages of equine asthma. Inhalation therapy led
    to significant reductions in clinical scores. MMP-2, MMP-9 and TIMP- 2 concentrations were reduced significantly immediately, and all MMP and TIMP concentrations 6 weeks after therapy.
    Discussion: In equine asthma, overexpression of MMPs contributes to pathological tissue destruction, while TIMPs counteract MMPs with
    overexpression leading to fibrosis formation. The results of this study show that CpG-ODN inhalation is an effective therapy to address a misbalance in equine asthma.
    Conclusions: Misbalance of elastinolytic activity was positively influenced by CpG-ODN inhalation for at least 6 post therapy, which may
    reduce the remodeling of the extracellular matrix. Further studies should evaluate this effect in comparison to glucocorticoid inhalation therapy.
    Significance: CpG-ODN inhalation may be an effective therapy in prevention of pulmonary fibrosis formation in equine asthma.