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    New real time and conventional RT-PCRs for updated molecular diagnosis of infectious bronchitis virus infection (IBV) in chickens in Egypt associated with frequent co-infections with avian influenza and Newcastle Disease viruses (2017)

    Art
    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Autoren
    Naguib, Mahmoud M
    El-Kady, Magdy F
    Lüschow, Dörte (WE 15)
    Hassan, Kareem E
    Arafa, Abdel-Satar
    El-Zanaty, Ali
    Hassan, Mohamed K
    Hafez, Hafez M (WE 15)
    Grund, Christian
    Harder, Timm C
    Quelle
    Journal of Virological Methods; 245 — S. 19–27
    ISSN: 0166-0934
    Sprache
    Englisch
    Verweise
    DOI: 10.1016/j.jviromet.2017.02.018
    Pubmed: 28336367
    Kontakt
    Institut für Geflügelkrankheiten

    Königsweg 63
    14163 Berlin
    Tel.+49 30 838 62676 Fax.+49 30 838 62690
    email:gefluegelkrankheiten@vetmed.fu-berlin

    Abstract / Zusammenfassung

    In Egypt, currently two geographically restricted genotypes of the infectious bronchitis coronavirus (IBV) are circulating with detrimental effects for poultry industry. A sensitive real-time RT-PCR assay targeting the IBV nucleocapsid gene (N) was developed to screen clinical samples for presence of IBV. Conventional RT-PCRs amplifying hypervariable regions (HVRs 1-2 and 3) of the IBV S1 gene were developed and amplificates used for nucleotide sequence-based typing of IBV field strains in Egyptian chickens directly from clinical samples.