Publikationsdatenbank

New real time and conventional RT-PCRs for updated molecular diagnosis of infectious bronchitis virus infection (IBV) in chickens in Egypt associated with frequent co-infections with avian influenza and Newcastle Disease viruses (2017)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Naguib, Mahmoud M

El-Kady, Magdy F

Lüschow, Dörte (WE 15)

Hassan, Kareem E

Arafa, Abdel-Satar

El-Zanaty, Ali

Hassan, Mohamed K

Hafez, Hafez M (WE 15)

Grund, Christian

Harder, Timm C

Quelle

Journal of Virological Methods

Bandzählung: 245

Seiten: 19 – 27

ISSN: 0166-0934

Sprache

Englisch

Verweise

DOI: 10.1016/j.jviromet.2017.02.018

Pubmed: 28336367

Kontakt

Nutztierklinik: Abteilung Geflügel

Königsweg 63
14163 Berlin
+49 30 838 62676
gefluegelkrankheiten@vetmed.fu-berlin.de

Abstract / Zusammenfassung

In Egypt, currently two geographically restricted genotypes of the infectious bronchitis coronavirus (IBV) are circulating with detrimental effects for poultry industry. A sensitive real-time RT-PCR assay targeting the IBV nucleocapsid gene (N) was developed to screen clinical samples for presence of IBV. Conventional RT-PCRs amplifying hypervariable regions (HVRs 1-2 and 3) of the IBV S1 gene were developed and amplificates used for nucleotide sequence-based typing of IBV field strains in Egyptian chickens directly from clinical samples.