Fachbereich Veterinärmedizin



    Altered Cytokine Expression and Barrier Properties after In Vitro Infection of Porcine Epithelial Cells with Enterotoxigenic Escherichia coli and Probiotic Enterococcus faecium (2017)

    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Kern, Martina (WE 2)
    Günzel, Dorothee
    Aschenbach, Jörg R (WE 2)
    Tedin, Karsten (WE 7)
    Bondzio, Angelika (WE 3)
    Lodemann, Ulrike (WE 2)
    Mediators of Inflammation; 2017 — S. 1–14
    ISSN: 0962-9351
    DOI: 10.1155/2017/2748192
    Pubmed: 28607532
    Institut für Mikrobiologie und Tierseuchen

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    Abstract / Zusammenfassung

    The aim of the present study was to elucidate the effects of the probiotic feed additive Enterococcus faecium NCIMB 10415 (E. faecium) on porcine jejunal epithelial cells (IPEC-J2) during an in vitro challenge with enterotoxigenic Escherichia coli (ETEC). Cells were incubated with E. faecium, ETEC, or both, and the effects on barrier function and structure and intra- and intercellular signaling were determined. Coincubation with E. faecium abolished the ETEC-induced decrease in transepithelial resistance (Rt) (p ≤ 0.05). No differences were seen in the expression levels of the intercellular connecting tight junction proteins examined. However, for the first time, a reorganization of the monolayer was observed in ETEC-infected cells but not in coincubated cells. ETEC induced an increase in cytotoxicity that was prevented by coincubation (p ≤ 0.05), whereas apoptosis rates were not affected by bacterial treatment. ETEC increased the mRNA expression and release of proinflammatory cytokines TNF-α, IL-1α, and IL-6 which could be prevented by coincubation for TNF-α mRNA expression and IL-6 protein (p ≤ 0.05). Likewise, cAMP concentrations elevated by ETEC were reduced in coincubated cells (p ≤ 0.05). These findings indicate a protective effect of the probiotic E. faecium on inflammatory responses during infection with ETEC.