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Clinic of Small Animals, Faculty of Veterinary Medicine, Freie Universität Berlin, Berlin, Berlin, Germany
Blood transfusions have become an integral part of feline intensive care treatment. For patient safety blood typing is an important pretransfusion test.
Objective of this prospective study was evaluation of an automated feline cartridge blood typing method (QuickVet®/RapidVet®(QVRV) SMB, Farum, Denmark) and comparison of results with slide and tube (reference) methods (not commercially available). Samples with discordant results were additionally tested with Alvedia® (Quick Test A+B, Alvedia, Limonest, France). To determine precision, blood typing of 3 A and B samples were repeated 5 times within 24 hours and over 5 consecutive days.
83 EDTA-anticoagulated blood samples routinely collected for diagnostic purposes and 16 provided by outside laboratories were available. (One sample with severe hemolysis could not be typed with QVRV.) Slide/tube tests yielded the same results on all 98 samples. 78 samples were blood type A, 14 B and 6 AB. QVRV identified 75/78 of the A and 14/14 of the B samples. Three A samples with low hematocrit (≤ 10%) could only be typed after diluting them. Six AB samples gave discordant results compared to the reference method: 1 AB sample was typed as blood type A with QVRV (and Alvedia®) and 5 as B with QVRV (and AB with Alvedia®). All repeats yielded the same results. Based on all samples run, QVRV agreed in 90.8% with the reference method (exclusively for A and B cats, the agreement was 96.5%). Blood samples with type B should be confirmed by back typing and samples with type AB by tube method.