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    Misbalance of Elastinolytic Activity in Equine Asthma positively influenced by Cpg-Odn Inhalation Therapy (2017)

    Art
    Vortrag
    Autoren
    Gehlen, Heidrun
    Barton, Ann Kristin (WE 17)
    Shety, Tarek
    Geis, Sabine
    Klier, John
    Einspanier, Ralf
    Kongress
    10th Annual European College of Equine Internal Medicine Congress ECEIM´17 Budapest
    Budapest, 02. – 04.11.2017
    Quelle
    Journal of veterinary internal medicine; 32(2) — S. 874
    ISSN: 0891-6640
    Sprache
    Englisch
    Verweise
    URL (Volltext): https://onlinelibrary.wiley.com/doi/epdf/10.1111/jvim.15044
    Kontakt
    Klinik für Pferde, allgemeine Chirurgie und Radiologie

    Oertzenweg 19 b
    14163 Berlin
    +49 30 838 62299
    pferdeklinik@vetmed.fu-berlin.de

    Abstract / Zusammenfassung

    Objectives: Inhalation of immunostimulatory bacterial DNA segments (cytosine-phosphate-guanosine-oligodeoxynucleotides, CpG-
    ODN) has been shown to normalize clinical and cytologic parameters in severe equine asthma (recurrent airway obstruction, RAO). We hypothesized that CpG-ODN inhalation reduces the misbalance of elastinolytic activity in horses affected by RAO.
    Methods: 20 horses diagnosed as RAO by clinical examinations using a scoring system were included. All horses were treated with inhalative CpG-ODN for 14 days in 2-day intervals. Matrix-metalloproteinase (MMP-2/-9) and tissue inhibitors of metalloproteinases (TIMP-1/-2) concentrations were measured in tracheal aspirates using equine ELISA kits (USCN Life Science Inc.) before, immediately and 6 weeks after CpG-ODN inhalation.
    Results: MMP and TIMP concentrations correlated with the results of clinical scoring in all stages of equine asthma. Inhalation therapy led
    to significant reductions in clinical scores. MMP-2, MMP-9 and TIMP-2 concentrations were reduced significantly immediately, and all MMP and TIMP concentrations 6 weeks after therapy.
    Discussion: In equine asthma, overexpression of MMPs contributes to pathological tissue destruction, while TIMPs counteract MMPs with
    overexpression leading to fibrosis formation. The results of this study show that CpG-ODN inhalation is an effective therapy to address a misbalance in equine asthma.
    Conclusions: Misbalance of elastinolytic activity was positively influenced by CpG-ODN inhalation for at least 6 post therapy, which may reduce the remodeling of the extracellular matrix. Further studies should evaluate this effect in comparison to glucocorticoid inhalation therapy.
    Significance: CpG-ODN inhalation may be an effective therapy in prevention of pulmonary fibrosis formation in equine asthma.