Publikationsdatenbank

Misbalance of Elastinolytic Activity in Equine Asthma positively influenced by Cpg-Odn Inhalation Therapy (2017)

Art

Vortrag

Autoren

Gehlen, Heidrun

Barton, Ann Kristin (WE 17)

Shety, Tarek

Geis, Sabine

Klier, John

Einspanier, Ralf

Kongress

10th Annual European College of Equine Internal Medicine Congress ECEIM´17 Budapest

Budapest, 02. – 04.11.2017

Quelle

Journal of veterinary internal medicine; 32(2) — S. 874

ISSN: 0891-6640

Sprache

Englisch

Verweise

URL (Volltext): https://onlinelibrary.wiley.com/doi/epdf/10.1111/jvim.15044

Kontakt

Klinik für Pferde, allgemeine Chirurgie und Radiologie

Oertzenweg 19 b
14163 Berlin
+49 30 838 62299
pferdeklinik@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Objectives: Inhalation of immunostimulatory bacterial DNA segments (cytosine-phosphate-guanosine-oligodeoxynucleotides, CpG-
ODN) has been shown to normalize clinical and cytologic parameters in severe equine asthma (recurrent airway obstruction, RAO). We hypothesized that CpG-ODN inhalation reduces the misbalance of elastinolytic activity in horses affected by RAO.
Methods: 20 horses diagnosed as RAO by clinical examinations using a scoring system were included. All horses were treated with inhalative CpG-ODN for 14 days in 2-day intervals. Matrix-metalloproteinase (MMP-2/-9) and tissue inhibitors of metalloproteinases (TIMP-1/-2) concentrations were measured in tracheal aspirates using equine ELISA kits (USCN Life Science Inc.) before, immediately and 6 weeks after CpG-ODN inhalation.
Results: MMP and TIMP concentrations correlated with the results of clinical scoring in all stages of equine asthma. Inhalation therapy led
to significant reductions in clinical scores. MMP-2, MMP-9 and TIMP-2 concentrations were reduced significantly immediately, and all MMP and TIMP concentrations 6 weeks after therapy.
Discussion: In equine asthma, overexpression of MMPs contributes to pathological tissue destruction, while TIMPs counteract MMPs with
overexpression leading to fibrosis formation. The results of this study show that CpG-ODN inhalation is an effective therapy to address a misbalance in equine asthma.
Conclusions: Misbalance of elastinolytic activity was positively influenced by CpG-ODN inhalation for at least 6 post therapy, which may reduce the remodeling of the extracellular matrix. Further studies should evaluate this effect in comparison to glucocorticoid inhalation therapy.
Significance: CpG-ODN inhalation may be an effective therapy in prevention of pulmonary fibrosis formation in equine asthma.