Fachbereich Veterinärmedizin



    Misbalance of Elastinolytic Activity in Equine Asthma positively influenced by Cpg-Odn Inhalation Therapy (2017)

    Gehlen, Heidrun
    Barton, Ann Kristin (WE 17)
    Shety, Tarek
    Geis, Sabine
    Klier, John
    Einspanier, Ralf
    10th Annual European College of Equine Internal Medicine Congress ECEIM´17 Budapest
    Budapest, 02. – 04.11.2017
    Journal of veterinary internal medicine; 32(2) — S. 874
    ISSN: 0891-6640
    URL (Volltext): https://onlinelibrary.wiley.com/doi/epdf/10.1111/jvim.15044
    Klinik für Pferde, allgemeine Chirurgie und Radiologie

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    14163 Berlin
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    Abstract / Zusammenfassung

    Objectives: Inhalation of immunostimulatory bacterial DNA segments (cytosine-phosphate-guanosine-oligodeoxynucleotides, CpG-
    ODN) has been shown to normalize clinical and cytologic parameters in severe equine asthma (recurrent airway obstruction, RAO). We hypothesized that CpG-ODN inhalation reduces the misbalance of elastinolytic activity in horses affected by RAO.
    Methods: 20 horses diagnosed as RAO by clinical examinations using a scoring system were included. All horses were treated with inhalative CpG-ODN for 14 days in 2-day intervals. Matrix-metalloproteinase (MMP-2/-9) and tissue inhibitors of metalloproteinases (TIMP-1/-2) concentrations were measured in tracheal aspirates using equine ELISA kits (USCN Life Science Inc.) before, immediately and 6 weeks after CpG-ODN inhalation.
    Results: MMP and TIMP concentrations correlated with the results of clinical scoring in all stages of equine asthma. Inhalation therapy led
    to significant reductions in clinical scores. MMP-2, MMP-9 and TIMP-2 concentrations were reduced significantly immediately, and all MMP and TIMP concentrations 6 weeks after therapy.
    Discussion: In equine asthma, overexpression of MMPs contributes to pathological tissue destruction, while TIMPs counteract MMPs with
    overexpression leading to fibrosis formation. The results of this study show that CpG-ODN inhalation is an effective therapy to address a misbalance in equine asthma.
    Conclusions: Misbalance of elastinolytic activity was positively influenced by CpG-ODN inhalation for at least 6 post therapy, which may reduce the remodeling of the extracellular matrix. Further studies should evaluate this effect in comparison to glucocorticoid inhalation therapy.
    Significance: CpG-ODN inhalation may be an effective therapy in prevention of pulmonary fibrosis formation in equine asthma.