Publikationsdatenbank

Porcine milk induces a strengthening of barrier function in porcine jejunal epithelium in vitro (2017)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Radloff, Judith (WE 2)

Zakrzewski, Silke S (WE 2)

Pieper, Robert (WE 4)

Markov, Alexander G

Amasheh, Salah (WE 2)

Quelle

Annals of the New York Academy of Sciences; 1397(1) — S. 110–118

ISSN: 1749-6632

Sprache

Englisch

Verweise

DOI: 10.1111/nyas.13340

Pubmed: 28445594

Kontakt

Institut für Veterinär-Physiologie

Oertzenweg 19 b
14163 Berlin
+49 30 838 62600
physiologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Milk contains a variety of components that have been shown to affect the expression and localization of epithelial tight junction proteins and therefore the intestinal barrier. Thus, we hypothesized that milk would have an effect on intestinal barrier properties, owing to effects on the tight junction in an intraspecies porcine intestinal in vitro model. Jejunal samples of piglets derived from different age groups were analyzed. Transepithelial electrical resistance was recorded employing the Ussing chamber technique. Porcine milk or predigested milk in buffer solution was added to the apical side, and effects were compared to untreated controls. Unidirectional paracellular flux measurements were performed using sodium fluorescein. Tight junction protein expression and localization were analyzed by immunoblotting and immunofluorescence microscopy. Incubation with milk or predigested milk led to an increase in transepithelial electrical resistance, while paracellular permeability for sodium fluorescein did not result in significant changes. Densitometric analysis of immunoblot signals did not show significant alterations in claudin expression, but a reduction of claudin signals in apicolateral membrane compartments in both approaches became apparent via immunohistology. The functional effect might reflect a physiological protective mechanism, when offspring exclusively rely on their mother's milk and are exposed to a plethora of potentially barrier-perturbing factors.