+49 30 838 51845
Background and objectives: The most frequent plasmidic encoded AmpC enzyme is CMY-2. It is produced by ca. 1% and ca. 30% of the third-generation cephalosporin-resistant Escherichia coli isolated from humans and poultry, respectively. In this study we are comparing plasmids from human, meat products and livestock animals from Germany, by whole-genome sequencing.
Materials and Methods: Genomic DNA of 168 CMY-2 positive E. coli from different sources (humans n=51, healthy broilers n=51, chicken meat n=56, turkey meat n=7, diseased pigs/chickens n=5,) was extracted and sequenced using the Illumina® MiSeq platform. Phylogenetic markers, such as plasmid multi-locus sequence type and plasmid replicon types were identified, as well as mobile genetic elements and whole plasmid comparisons were conducted.
Results: Plasmids carrying blaCMY-2 were identified in 145 of the 168 sequenced isolates; in the rest of the isolates blaCMY-2 was integrated in the chromosome. Plasmids of the replicon type IncI1 (n=64) and IncK (n=76) were the most prevalent ones and among these, identical plasmid sequences (nucleotide identity 96-99%) present in all habitats, were observed. Further four IncA/C plasmids were identified.
Conclusions: The results showed highly related plasmids from different reservoirs. This indicates a possible plasmid-mediated spread and zoonotic potential of blaCMY-2 carrying plasmids across the E. coli host populations.