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    Comparisons of AmpC-beta-lactamase CMY-2 encoding plasmids from Escherichia coli from humans, livestock and food in Germany (2017)

    Art
    Poster
    Autoren
    Pietsch, M
    Irrgang, A.
    Roschanski, N. (WE 10)
    Brenner Michael, G. (WE 7)
    Käsbohrer, A.
    Schwarz, S. (WE 7)
    Rösler, U. (WE 10)
    Pfeifer, Y.
    Werner, G.
    Kongress
    National Symposium on Zoonoses Research 2017
    12. – 13.10.2017
    Quelle
    National Symposium on Zoonoses Research 2017 — German Research Platform for Zoonoses (Hrsg.)
    — S. 152–153
    Sprache
    Englisch
    Verweise
    URL (Volltext): http://www.zoonosen.net/Desktopmodules/Bring2Mind/DMX/Download.aspx?EntryId=31102&PortalId=24
    Kontakt
    Institut für Tier- und Umwelthygiene

    Robert-von-Ostertag-Str. 7-13
    Gebäude 35
    14169 Berlin
    +49 30 838 51845
    tierhygiene@vetmed.fu-berlin.de

    Abstract / Zusammenfassung

    Background and objectives: The most frequent plasmidic encoded AmpC enzyme is CMY-2. It is produced by ca. 1% and ca. 30% of the third-generation cephalosporin-resistant Escherichia coli isolated from humans and poultry, respectively. In this study we are comparing plasmids from human, meat products and livestock animals from Germany, by whole-genome sequencing.
    Materials and Methods: Genomic DNA of 168 CMY-2 positive E. coli from different sources (humans n=51, healthy broilers n=51, chicken meat n=56, turkey meat n=7, diseased pigs/chickens n=5,) was extracted and sequenced using the Illumina® MiSeq platform. Phylogenetic markers, such as plasmid multi-locus sequence type and plasmid replicon types were identified, as well as mobile genetic elements and whole plasmid comparisons were conducted.
    Results: Plasmids carrying blaCMY-2 were identified in 145 of the 168 sequenced isolates; in the rest of the isolates blaCMY-2 was integrated in the chromosome. Plasmids of the replicon type IncI1 (n=64) and IncK (n=76) were the most prevalent ones and among these, identical plasmid sequences (nucleotide identity 96-99%) present in all habitats, were observed. Further four IncA/C plasmids were identified.
    Conclusions: The results showed highly related plasmids from different reservoirs. This indicates a possible plasmid-mediated spread and zoonotic potential of blaCMY-2 carrying plasmids across the E. coli host populations.