Publication Database

Comparisons of AmpC-beta-lactamase CMY-2 encoding plasmids from Escherichia coli from humans, livestock and food in Germany (2017)

Art

Poster

Autoren

Pietsch, M

Irrgang, A.

Roschanski, N. (WE 10)

Brenner Michael, G. (WE 7)

Käsbohrer, A.

Schwarz, S. (WE 7)

Rösler, U. (WE 10)

Pfeifer, Y.

Werner, G.

Kongress

National Symposium on Zoonoses Research 2017

12. – 13.10.2017

Quelle

National Symposium on Zoonoses Research 2017 — German Research Platform for Zoonoses (Hrsg.)

— S. 152–153

Sprache

Englisch

Verweise

URL (Volltext): http://www.zoonosen.net/Desktopmodules/Bring2Mind/DMX/Download.aspx?EntryId=31102&PortalId=24

Kontakt

Institut für Tier- und Umwelthygiene

Robert-von-Ostertag-Str. 7-13
14169 Berlin
+49 30 838 51845
tierhygiene@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Background and objectives: The most frequent plasmidic encoded AmpC enzyme is CMY-2. It is produced by ca. 1% and ca. 30% of the third-generation cephalosporin-resistant Escherichia coli isolated from humans and poultry, respectively. In this study we are comparing plasmids from human, meat products and livestock animals from Germany, by whole-genome sequencing.
Materials and Methods: Genomic DNA of 168 CMY-2 positive E. coli from different sources (humans n=51, healthy broilers n=51, chicken meat n=56, turkey meat n=7, diseased pigs/chickens n=5,) was extracted and sequenced using the Illumina® MiSeq platform. Phylogenetic markers, such as plasmid multi-locus sequence type and plasmid replicon types were identified, as well as mobile genetic elements and whole plasmid comparisons were conducted.
Results: Plasmids carrying blaCMY-2 were identified in 145 of the 168 sequenced isolates; in the rest of the isolates blaCMY-2 was integrated in the chromosome. Plasmids of the replicon type IncI1 (n=64) and IncK (n=76) were the most prevalent ones and among these, identical plasmid sequences (nucleotide identity 96-99%) present in all habitats, were observed. Further four IncA/C plasmids were identified.
Conclusions: The results showed highly related plasmids from different reservoirs. This indicates a possible plasmid-mediated spread and zoonotic potential of blaCMY-2 carrying plasmids across the E. coli host populations.