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    Colonisation of broiler chickens with ESBL-/AmpC- producing E.coli using s seeder- bird model and detection of in vivo transformants (2017)

    Art
    Vortrag
    Autoren
    Robè, Caroline (WE 10)
    Blasse, Anja (WE 10)
    Dähre, Katrin (WE 10)
    Rösler, Uwe (WE 10)
    Günther, Sebastian (WE 10)
    Kongress
    National Symposium on Zoonoses Research 2017
    12. – 13.10.2017
    Quelle
    National Symposium on Zoonoses Research 2017 — German Research Platform for Zoonoses (Hrsg.)
    — S. 76
    Sprache
    Englisch
    Verweise
    URL (Volltext): http://www.zoonosen.net/Desktopmodules/Bring2Mind/DMX/Download.aspx?EntryId=31102&PortalId=24
    Kontakt
    Institut für Tier- und Umwelthygiene

    Robert-von-Ostertag-Str. 7-13
    Gebäude 35
    14169 Berlin
    +49 30 838 51845
    tierhygiene@vetmed.fu-berlin.de

    Abstract / Zusammenfassung

    Background and objectives: Colonisation of broiler with ESBL- and AmpC- producing Enterobacteriaceae is well known, resulting in the possibility of transfer to humans either by a close contact to broiler flocks or through contaminated retail meat. To examine potential intervention strategies regarding hygiene- and management measures a broiler colonisation model was established.
    Materials and methods: Broiler were conventionally housed in to identify the oral colonisation dose and to establish a seeder- bird- colonisation model close to real farming conditions. ESBL-/ AmpC- negative day- old broiler were orally co-infected on the third day of life with one ESBL- and one AmpC- producing E. coli strain. Colonisation success was proven by cloacal swabs over a period of 14 to 35 days and a final section. By using different selective media it was possible to verify in vivo plasmid transfer between the two strains.
    Results: An oral infection dose of 102 cfu per animal is sufficient to colonise broiler 24 h post infection up to the end of the trial. A relation of 1:5 infected (seeder) to susceptible animals is adequate to colonise the complete flock. In addition transformants carrying both resistant plasmids were detectable after 72 h p.i.
    Conclusion: Given the low infection dose and seeder numbers the spread of ESBL-/ AmpC- producers in conventional farms is not surprising. We will test hygiene- and management interventions using this model as a possible approach for reduction.