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    Esbl-/ AmpC-producing Enterobacteriaceae in broiler fattening farms after cleaning and disinfection - identification of critical control points (2017)

    Art
    Vortrag
    Autoren
    Dähre, Katrin (WE 10)
    Robè, Caroline (WE 10)
    Blasse, Anja (WE 10)
    Friese, Anika (WE 10)
    Rösler, Uwe (WE 10)
    Kongress
    National Symposium on Zoonoses Research 2017
    12. – 13.10.2017
    Quelle
    National Symposium on Zoonoses Research 2017 — German Research Platform for Zoonoses (Hrsg.)
    — S. 74
    Sprache
    Englisch
    Verweise
    URL (Volltext): http://www.zoonosen.net/Desktopmodules/Bring2Mind/DMX/Download.aspx?EntryId=31102&PortalId=24
    Kontakt
    Institut für Tier- und Umwelthygiene

    Robert-von-Ostertag-Str. 7-13
    Gebäude 35
    14169 Berlin
    +49 30 838 51845
    tierhygiene@vetmed.fu-berlin.de

    Abstract / Zusammenfassung

    Background and objectives: Broiler farms are known as reservoirs for ESBL-/AmpC-producing Enterobacteriaceae. The reduction of these bacteria should be achieved by cleaning and disinfection procedures (C&D) between different fattening periods. Aiming on the elucidation of possible niches for the survival of ESBL-/AmpC-producing Enterobacteriaceae and the identification of critical control points (CCP) we investigated five broiler farms before and after C&D.
    Material and methods: Five ESBL-/AmpC-positive broiler farms were identified by an initial screening of the current fattening flocks. Following, these farms were investigated intensively by taking gauze swabs and boot swabs after C&D from both the inside and the outside of the farms. ESBL-/AmpC-producing Enterobacteriaceae were isolated and examined for their bacterial species, phylogroup and resistance genes. Additionally, all samples were investigated for Enterococci as an indicator for faecal contamination.
    Results: In our study, both investigated microorganisms survived the C&D in 4/5 farms. Thereby, ESBL-/AmpC- producers were only detected in samples that simultaneously were positive for Enterococci. Isolates detected at the initial screening and after C&D of the same farm showed equal molecular characteristics which will be further investigated by PFGE in this ongoing study.
    Conclusion: ESBL-/AmpC-producing Enterobacteriaceae can survive C&D and therefore pose a risk for the colonisation of consecutively fattening flocks.