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Background and objectives: Broiler farms are known as reservoirs for ESBL-/AmpC-producing Enterobacteriaceae. The reduction of these bacteria should be achieved by cleaning and disinfection procedures (C&D) between different fattening periods. Aiming on the elucidation of possible niches for the survival of ESBL-/AmpC-producing Enterobacteriaceae and the identification of critical control points (CCP) we investigated five broiler farms before and after C&D.
Material and methods: Five ESBL-/AmpC-positive broiler farms were identified by an initial screening of the current fattening flocks. Following, these farms were investigated intensively by taking gauze swabs and boot swabs after C&D from both the inside and the outside of the farms. ESBL-/AmpC-producing Enterobacteriaceae were isolated and examined for their bacterial species, phylogroup and resistance genes. Additionally, all samples were investigated for Enterococci as an indicator for faecal contamination.
Results: In our study, both investigated microorganisms survived the C&D in 4/5 farms. Thereby, ESBL-/AmpC- producers were only detected in samples that simultaneously were positive for Enterococci. Isolates detected at the initial screening and after C&D of the same farm showed equal molecular characteristics which will be further investigated by PFGE in this ongoing study.
Conclusion: ESBL-/AmpC-producing Enterobacteriaceae can survive C&D and therefore pose a risk for the colonisation of consecutively fattening flocks.