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    ESBL-Plasmids Interfere with Biofilm Formation, Competitive Adhesion and Serum Resistance (2017)

    Art
    Vortrag
    Autoren
    Schaufler, K. (WE 7)
    Ranjan, A.
    Semmler, T. (WE 7)
    Wieler, L.H. (WE 7)
    Ewers, C.
    Pickard, D.J.
    Guenther, S. (WE 10)
    Kongress
    XVIII ISAH Congress 2017
    Mazatlan, Sinaloa, Mexico, 19. – 23.03.2017
    Quelle
    XVIII ISAH Congress 2017 March 19 – 23, 2017, Mazatlán, Sinaloa, Mexico PROCEEDINGS of the XVIII International Congress of the International Society for Animal Hygiene “International Co-operation and Solidarity in Animal Hygiene towards One Health”
    — S. 330
    Sprache
    Englisch
    Verweise
    URL (Volltext): https://jmonnetblog.files.wordpress.com/2017/04/xviii-isah-congress-2017.pdf
    Kontakt
    Institut für Tier- und Umwelthygiene

    Robert-von-Ostertag-Str. 7-13
    Gebäude 35
    14169 Berlin
    +49 30 838 51845
    tierhygiene@vetmed.fu-berlin.de

    Abstract / Zusammenfassung

    INTRODUCTION: ESBL-producing E. coli have become abundant all over the world and especially clonal lineages like ST131 and ST648 are of utmost importance. This study investigated the influence of ESBL-plasmids on non-resistance factors like biofilm formation, competition with commensals, competitive adhesion, serum resistance and motility of the host bacteria.
    MATERIAL AND METHODS: Seven triplets of ESBL-carrying wild-type (WT) strains, their corresponding ESBL-plasmid-“cured” variant (PCV) and a complementary ESBLcarrying transformant (T) where analyzed in long-term colony, competition assays with commensals in co-cultures, competitive adhesion on IPEC-J2 cell lines, serum resistance, swimming motility, Biolog phenotypic microarrays, whole-genome sequence and RNA-sequence analysis.
    RESULTS:
    For some of the triplets we detected enhanced curli and/or cellulose production and a reduced swimming capacity of the WT and T strain compared to their PCV. RNA sequencing revealed the chromosomally-encoded csgD-pathway as a key factor involved. Biolog results pointed towards a similar metabolic behavior of WT, PCV and T. In addition, ESBL plasmids played protective role against serum bactericidal activity and did not no generally present a burden to the host in competition assays against commensal E. coli in co-cultures and adhesion assays on epithelial cell lines. For some of the strains carriage of ESBL-plasmids was even advantageous for competition.
    CONCLUSIONS:
    Our phenotypic and RNA sequencing results clearly indicate an influence of ESBLplasmids on non-resistance factors important for virulence and survival of the pathogenic strains, presumably contributing to their pandemic success.