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    Retrospective survey of mcr-1 and mcr-2 in German pig-fattening farms, 2011-2012 (2017)

    Art
    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Autoren
    Roschanski, Nicole (WE 10)
    Falgenhauer, Linda
    Grobbel, Mirjam
    Guenther, Sebastian (WE 10)
    Kreienbrock, Lothar
    Imirzalioglu, Can
    Roesler, Uwe (WE 10)
    Forschungsprojekt
    Langzeit-Monitoring von ESBL-bildenden und Fluor-Chinolon-resistenten Enterobacteriaceen in Nutztierhaltungen und deren Umgebung
    Quelle
    International journal of antimicrobial agents; 50(2) — S. 266–271
    ISSN: 0924-8579
    Sprache
    Englisch
    Verweise
    DOI: 10.1016/j.ijantimicag.2017.03.007
    Pubmed: 28545990
    Kontakt
    Institut für Tier- und Umwelthygiene

    Robert-von-Ostertag-Str. 7-13
    Gebäude 35
    14169 Berlin
    +49 30 838 51845
    tierhygiene@vetmed.fu-berlin.de

    Abstract / Zusammenfassung

    In November 2015, the first plasmid-encoded colistin resistance gene, mcr-1, was described in animals and in humans in China. Subsequently, a multitude of further studies was performed and quite recently the global spread of mcr-1 as well as the occurrence of a new gene variant, mcr-2, was reported. To obtain an overview of the occurrence of the colistin resistance genes mcr-1 and mcr-2 in German pig farms, a retrospective study, including 436 boot swab and pooled faecal samples collected from 58 pig-fattening farms throughout Germany, was performed. Whilst mcr-2 was not detected, the presence of mcr-1 was confirmed in 43 Escherichia coli isolates from 15 farms, indicating that the mcr-1 gene was present in 9.9% of the analysed samples and 25.9% of the investigated pig farms. Subsequent characterisation of the isolates showed colistin minimum inhibitory concentrations (MICs) of 4-8 µg/mL, with most isolates being resistant to several antibiotics including cephalosporins and/or fluoroquinolones. Pulsed-field gel electrophoresis (PFGE) showed great heterogeneity among the tested mcr-1-positive isolates. However, further analyses of 15 selected E. coli isolates (one per mcr-1-positive farm) indicated that the colistin resistance genes were predominantly located on IncX4 plasmids, highly similar to a plasmid initially isolated from an E. coli derived from a human patient in Brazil. The results described herein support the already expressed concern for public health and further underline the need for monitoring programmes in veterinary practice as well as in human medicine.