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    Microsatellites Within the Feline Androgen Receptor are Suitable for X Chromosome-linked Clonality Testing in Archival Material (2017)

    Art
    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Autoren
    Farwick, Nadine M
    Klopfleisch, Robert (WE 12)
    Gruber, Achim D (WE 12)
    Weiss, Alexander Th A
    Quelle
    Journal of feline medicine and surgery; 19(4) — S. 454–460
    ISSN: 1098-612x
    Sprache
    Englisch
    Verweise
    DOI: 10.1177/1098612X16634386
    Pubmed: 26919893
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    Abstract / Zusammenfassung

    Objectives A hallmark of neoplasms is their origin from a single cell; that is, clonality. Many techniques have been developed in human medicine to utilise this feature of tumours for diagnostic purposes. One approach is X chromosome-linked clonality testing using polymorphisms of genes encoded by genes on the X chromosome. The aim of this study was to determine if the feline androgen receptor gene was suitable for X chromosome-linked clonality testing. Methods The feline androgen receptor gene was characterised and used to test clonality of feline lymphomas by PCR and polyacrylamide gel electrophoresis, using archival formalin-fixed, paraffin-embedded material. Results Clonality of the feline lymphomas under study was confirmed and the gene locus was shown to represent a suitable target in clonality testing. Conclusions and relevance Because there are some pitfalls of using X chromosome-linked clonality testing, further studies are necessary to establish this technique in the cat.