Publikationsdatenbank

Microsatellites Within the Feline Androgen Receptor are Suitable for X Chromosome-linked Clonality Testing in Archival Material (2017)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Farwick, Nadine M

Klopfleisch, Robert (WE 12)

Gruber, Achim D (WE 12)

Weiss, Alexander Th A

Quelle

Journal of feline medicine and surgery : official journal of the European Society of Feline Medicine

Bandzählung: 19

Heftzählung: 4

Seiten: 454 – 460

ISSN: 1098-612x

Sprache

Englisch

Verweise

DOI: 10.1177/1098612X16634386

Pubmed: 26919893

Kontakt

Institut für Tierpathologie

Robert-von-Ostertag-Str. 15
14163 Berlin
+49 30 838 62450
pathologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Objectives A hallmark of neoplasms is their origin from a single cell; that is, clonality. Many techniques have been developed in human medicine to utilise this feature of tumours for diagnostic purposes. One approach is X chromosome-linked clonality testing using polymorphisms of genes encoded by genes on the X chromosome. The aim of this study was to determine if the feline androgen receptor gene was suitable for X chromosome-linked clonality testing. Methods The feline androgen receptor gene was characterised and used to test clonality of feline lymphomas by PCR and polyacrylamide gel electrophoresis, using archival formalin-fixed, paraffin-embedded material. Results Clonality of the feline lymphomas under study was confirmed and the gene locus was shown to represent a suitable target in clonality testing. Conclusions and relevance Because there are some pitfalls of using X chromosome-linked clonality testing, further studies are necessary to establish this technique in the cat.