Fachbereich Veterinärmedizin



    TSLP is a Direct Trigger for T cell Migration in Filaggrin-Deficient Skin Equivalents (2017)

    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Wallmeyer, Leonie
    Dietert, Kristina (WE 12)
    Sochorová, Michaela
    Gruber, Achim D (WE 12)
    Kleuser, Burkhard
    Vávrová, Kateřina
    Hedtrich, Sarah
    Scientific reports; 7(1) — S. 774
    ISSN: 2045-2322
    DOI: 10.1038/s41598-017-00670-2
    Pubmed: 28377574
    Institut für Tierpathologie

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    Abstract / Zusammenfassung

    Mutations in the gene encoding for filaggrin (FLG) are major predisposing factors for atopic dermatitis (AD). Besides genetic predisposition, immunological dysregulations considerably contribute to its pathophysiology. For example, thymic stromal lymphopoietin (TSLP) is highly expressed in lesional atopic skin and significantly contributes to the pathogenesis of AD by activating dendritic cells that then initiate downstream effects on, for example, T cells. However, little is known about the direct interplay between TSLP, filaggrin-deficient skin and other immune cells such as T lymphocytes. In the present study, FLG knockdown skin equivalents, characterised by intrinsically high TSLP levels, were exposed to activated CD4(+) T cells. T cell exposure resulted in an inflammatory phenotype of the skin equivalents. Furthermore, a distinct shift from a Th1/Th17 to a Th2/Th22 profile was observed following exposure of T cells to filaggrin-deficient skin equivalents. Interestingly, TSLP directly stimulated T cell migration exclusively in filaggrin-deficient skin equivalents even in the absence of dendritic cells, indicating a hitherto unknown role of TSLP in the pathogenesis of AD.