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    Antimicrobial susceptibility testing of Arcobacter butzleri: development and application of a new protocol for broth microdilution (2017)

    Art
    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Autoren
    Riesenberg, Anne
    Frömke, Cornelia
    Stingl, Kerstin
    Feßler, Andrea T. (WE 7)
    Gölz, Greta (WE 8)
    Glocker, Erik-Oliver
    Kreienbrock, Lothar
    Klarmann, Dieter
    Werckenthin, Christiane
    Schwarz, Stefan
    Quelle
    The Journal of antimicrobial chemotherapy — S. 1–6
    ISSN: 0305-7453
    Sprache
    Englisch
    Verweise
    URL (Volltext): https://academic.oup.com/jac/article/doi/10.1093/jac/dkx211/3959863/Antimicrobial-susceptibility-testing-of-Arcobacter
    DOI: 10.1093/jac/dkx211
    Kontakt
    Institut für Lebensmittelsicherheit und -hygiene

    Königsweg 69
    14163 Berlin
    Tel.+49 30 838 62550 Fax.+49 30 838 46029
    email:lebensmittelhygiene@vetmed.fu-berlin.de

    Abstract / Zusammenfassung

    Objectives: To develop a standard reference broth microdilution method for antimicrobial susceptibility testing (AST) of Arcobacter butzleri. The protocol was subsequently applied to a collection of A. butzleri isolates from different sources.

    Methods: Broth microdilution susceptibility testing was performed on eight A. butzleri isolates in three media: non-supplemented CAMHB, CAMHB + 2% FBS and CAMHB + 5% FBS. The MIC values were read after 24 and 48 h of incubation at 35 ± 2 °C in ambient air. A logistic regression model was used to determine the combination of medium and incubation time yielding the most homogeneous results. Subsequently, the protocol was applied to 65 A. butzleri isolates to determine their MICs of 31 antimicrobial agents.

    Results: The statistical analysis revealed that the most homogeneous MIC values were obtained with CAMHB + 5% FBS and reading of MIC values after 24 h of incubation. The standardized method was successful for AST of all 65 A. butzleri isolates. MIC values were distributed unimodally for most antimicrobial agents. However, one field isolate showed elevated MIC values of gentamicin, streptomycin, tetracycline and trimethoprim/sulfamethoxazole.

    Conclusions: This study presents a new protocol for AST of A. butzleri by broth microdilution and shows the distribution of MIC values of 31 antimicrobial agents for a collection of A. butzleri isolates from different origins.