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    Occurrence of Methicillin Resistant Staphylococcus Aureus (MRSA) on the ocular surfaces of horses undergoing ophthalmic manipulation (2017)

    Art
    Poster
    Autoren
    Tanawan, S. (WE 20)
    Lübke-Becker, A. (WE 7)
    Walther, B. (WE 7)
    Eule, J.C. (WE 20)
    Kongress
    ECVO Meeting
    Estoril, Portugal, 19. – 21.05.2017
    Quelle
    Veterinary Ophthalmology; 20(4) — S. E11
    ISSN: 1463-5216
    Sprache
    Englisch
    Verweise
    URL (Volltext): http://onlinelibrary.wiley.com/doi/10.1111/vop.12498/pdf
    DOI: 10.1111/vop.12498
    Kontakt
    Klinik für kleine Haustiere

    Oertzenweg 19 b
    Haus 1
    14163 Berlin
    +49 30 838 62356
    kleintierklinik@vetmed.fu-berlin.de

    Abstract / Zusammenfassung

    Purpose: Upon reviewing literature, information regarding occurrence and characteristics of antibiotic-resistant bacteria obtained from ocular surfaces of horses in Europe is scarce. The aim of this study was to determine the occurrence of methicillin resistant Staphylococcus aureus (MRSA) on ocular surfaces of horses undergoing ophthalmic manipulation (paracentesis, biopsy, surgery). Methods: Conjunctival swabs were collected from 47 horses (49 eyes) (group 1: n = 8 conjunctivitis/blepharitis, group 2: n = 9 keratitis, group 3: n = 32 uveitis). All specimens were immediately processed and identification and susceptibility testing of colonies suspected to be S. aureus was carried out using Vitek2 system (bioMerieux, Lyon) and the presence of MRSA was verified by PCR. Genetic relatedness of all MRSA isolates were investigated by pulsed-field gel electrophoresis (PFGE) and spa-typing. Result: MRSA
    was isolated from 6/49 (12.2%) samples. While none of the horses of the first group were found MRSA positive, 3/9 (30.0%) horses of the second and 3/32 (9.8%) of the third group showed MRSA positive swabs. PFGE analysis revealed a very close relatedness (>90%) of the six MRSA isolates, all assigned to spa-type t011, a spa type commonly associated with the European livestock-associated MRSA lineage of the clonal complex (CC) 398. Conclusion: The study demonstrated a high prevalence of MRSA on the ocular surfaces of equine ophthalmic patients – especially in those with keratitis (30%) – supporting the need for constant bacterial surveillance.