Publikationsdatenbank

Full-thickness skin constructs: endothelialized versus non-endothelialized:
A morphological comparison regarding epidermal differentiation (2016)

Art

Poster

Autoren

Khiao In, Maneenooch (WE 1)

Wallmeyer, L

Hedtrich, Sarah

Plendl, Johanna (WE 1)

Kaessmeyer, Sabine (WE 1)

Kongress

82. Jahrestagung der Deutschen Gesellschaft für experimentelle und klinische Pharmakologie und Toxikologie (DGPT) und 18. Jahrestagung der Klinischen Pharmakologie (VKliPha) in Zusammenarbeit mit der AGAH

Berlin, 29.02. – 03.03.2016

Quelle

Naunyn-Schmiedeberg's archives of pharmacology

Bandzählung: 389

Heftzählung: 1, Supplement 1

Seiten: S23 – S24

ISSN: 0028-1298

Sprache

Englisch

Verweise

URL (Volltext): https://link.springer.com/content/pdf/10.1007%2Fs00210-016-1213-y.pdf

DOI: 10.1007/s00210-016-1213-y

Kontakt

Institut für Veterinär-Anatomie

Koserstr. 20
14195 Berlin
+49 30 838 75784
anatomie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Introduction: Vascularization of the dermal equivalent of full-thickness skin constructs by endothelial cells is highly desirable, because such constructs closely mimic the architecture of real skin. Unfortunately, the realization of a capillary network in skin constructs is still difficult. In our study of full-thickness skin constructs, following the methodologies of Küchler et al. (2011), there were alterations in the epidermal differentiation after endothelialization of the dermal equivalent. The aim of this study wasto characterize these changes on a morphological level.
Material and methods: Non-endothelialized constructs (keratinocytes, fibroblasts) were prepared according to Küchler et al. (2011). To obtain endothelialized constructs, the dermal equivalent of the non-endothelialized constructs was enriched with endothelial cells. After two weeks of in vitro culture, the skin constructs were processed for quantitative as well as qualitative assessment by light and electron microscopy.
Results: Both types of skin construct developed all strata, i.e., stratum basale, spinosum, granulosum, corneum of a stratified soft-cornified epidermis, although the two constructs displayed differences in every stratum: Significantly more mitoses occurred in the epithelial germ layers of the endothelialized constructs (p=0.013). In addition, significantly more keratohyalin granules were counted within their stratum granulosum (p=0.010). 50% of the shapes of the spinous and the granulosum cells were irregular and these cells were separated by wide intercellular spaces. The typical epidermal lamellar bodies appeared in the endothelialized constructs more often than in the nonendothelialized ones. At the stratum granulosum – stratum corneum interface, no cohesion between the strata was present.
Conclusion: The endothelialization of the dermal equivalent caused changes in the epidermal layer of the endothelialized skin constructs that may be interpreted as features of excessive epidermal differentiation.