Fachbereich Veterinärmedizin



    Peptide-binding motifs of two common equine class I MHC molecules in Thoroughbred horses (2017)

    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Bergmann, Tobias (WE 3)
    Lindvall, Mikaela
    Moore, Erin
    Moore, Eugene
    Sidney, John
    Miller, Donald
    Tallmadge, Rebecca L
    Myers, Paisley T
    Malaker, Stacy A
    Shabanowitz, Jeffrey
    Osterrieder, Nikolaus (WE 5)
    Peters, Bjoern
    Hunt, Donald F
    Antczak, Douglas F
    Sette, Alessandro
    Immunogenetics; 69(5) — S. 351–358
    ISSN: 0093-7711
    DOI: 10.1007/s00251-017-0978-6
    Pubmed: 28315936
    Institut für Virologie

    Robert-von-Ostertag-Str. 7-13
    Gebäude 35
    14163 Berlin
    +49 30 838 51833

    Abstract / Zusammenfassung

    Quantitative peptide-binding motifs of MHC class I alleles provide a valuable tool to efficiently identify putative T cell epitopes. Detailed information on equine MHC class I alleles is still very limited, and to date, only a single equine MHC class I allele, Eqca-1*00101 (ELA-A3 haplotype), has been characterized. The present study extends the number of characterized ELA class I specificities in two additional haplotypes found commonly in the Thoroughbred breed. Accordingly, we here report quantitative binding motifs for the ELA-A2 allele Eqca-16*00101 and the ELA-A9 allele Eqca-1*00201. Utilizing analyses of endogenously bound and eluted ligands and the screening of positional scanning combinatorial libraries, detailed and quantitative peptide-binding motifs were derived for both alleles. Eqca-16*00101 preferentially binds peptides with aliphatic/hydrophobic residues in position 2 and at the C-terminus, and Eqca-1*00201 has a preference for peptides with arginine in position 2 and hydrophobic/aliphatic residues at the C-terminus. Interestingly, the Eqca-16*00101 motif resembles that of the human HLA A02-supertype, while the Eqca-1*00201 motif resembles that of the HLA B27-supertype and two macaque class I alleles. It is expected that the identified motifs will facilitate the selection of candidate epitopes for the study of immune responses in horses.