Fachbereich Veterinärmedizin



    Pathogen-reactive T helper cell analysis in the Pig (2017)

    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Ebner, Friederike (WE 6)
    Schwiertz, Patrycja (WE 6)
    Steinfelder, Svenja (WE 6)
    Pieper, Robert (WE 4)
    Zentek, Jürgen (WE 4)
    Schütze, Nicole
    Baums, Christoph G.
    Alber, Gottfried
    Geldhof, Peter
    Hartmann, Susanne (WE 6)
    Frontiers in immunology; 8(Art. 565) — S. 1–15
    ISSN: 1664-3224
    URL (Volltext): http://journal.frontiersin.org/article/10.3389/fimmu.2017.00565/full
    DOI: 10.3389/fimmu.2017.00565
    Institut für Immunologie

    Robert-von-Ostertag-Str. 7-13
    Gebäude 35
    14163 Berlin
    +49 30 838 51834

    Abstract / Zusammenfassung

    There is growing interest in studying host–pathogen interactions in human-relevant large animal models such as the pig. Despite the progress in developing immunological reagents for porcine T cell research, there is an urgent need to directly assess pathogen-specific T cells—an extremely rare population of cells, but of upmost importance in orchestrating the host immune response to a given pathogen. Here, we established that the activation marker CD154 (CD40L), known from human and mouse studies, identifies also porcine antigen-reactive CD4+ T lymphocytes. CD154 expression was upregulated early after antigen encounter and CD4+CD154+ antigen-reactive T cells coexpressed cytokines. Antigen-induced expansion and autologous restimulation enabled a time- and dose-resolved analysis of CD154 regulation and a significantly increased resolution in phenotypic profiling of antigen-responsive cells. CD154 expression identified T cells responding to staphylococcal Enterotoxin B superantigen stimulation as well as T cells responding to the fungus Candida albicans and T cells specific for a highly prevalent intestinal parasite, the nematode Ascaris suum during acute and trickle infection. Antigen-reactive T cells were further detected after immunization of pigs with a single recombinant bacterial antigen of Streptococcus suis only. Thus, our study offers new ways to study antigen-specific T lymphocytes in the pig and their contribution to host–pathogen interactions.