Fachbereich Veterinärmedizin



    Evaluation of Two Protocols Using Autologous Conditioned Serum for Intra-articular Therapy of Equine Osteoarthritisd - A Pilot Study Monitoring Cytokines and Cartilage-Specific Biomarkers (2016)

    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Lasarzik, Juliane (WE 17)
    Bondzio, Angelika (WE 3)
    Rettig, Matthias (WE 17)
    Estrada, Roberto (WE 17)
    Klaus, Christoph (WE 17)
    Ehrle, Anna
    Einspanier, Ralf (WE 3)
    Lischer, Christoph J (WE 17)
    Journal of Equine Veterinary Science — S. 1–8.e2
    ISSN: 0737-0806
    URL (Volltext): http://dx.doi.org/10.1016/j.jevs.2016.09.014
    DOI: 10.1016/j.jevs.2016.09.014
    Institut für Veterinär-Biochemie

    Oertzenweg 19 b
    14163 Berlin
    +49 30 838 62225

    Abstract / Zusammenfassung

    We hypothesised that shorter treatment intervals of intraarticular autologous conditioned serum (ACS) injections would more beneficially affect the synovial fluid (SF) concentrations of IL-1ra, IL-1β and cartilage biomarkers, compared with the traditional weekly treatment intervals in joints suffering from natural OA. In a randomised comparative study, 12 horses with OA were allocated to two groups (n = 6). The horses in group 1 received three intraarticular ACS injections at weekly intervals, whereas the horses in group 2 received three intraarticular ACS injections at two-day intervals. The levels of IL-1ra, IL-1β, CPII, C12C and CS 846 were determined in SF before and after ACS treatment using commercially available ELISA kits. The SF IL-1ra concentration 1 hour and 4 hours after ACS injection was significantly increased compared to baseline levels and decreased back to it within 48 hours. Comparing the SF IL-1ra, IL-1β, C12C, CS 846 and CP II levels before and 42 days after ACS treatment, group 2 showed a significant decrease in all parameters and an approximation on the levels in normal joints. These results indicate that the long-time effect of an ACS treatment given at two-day intervals is characterized by decreased SF IL1ra, IL-1β, C12C, CP II and CS 846 concentrations, which might indicate an improvement in joint inflammation and cartilage degrading processes . Further investigations with greater sample sizes have to prove if the two-day treatment interval is preferable to the widely used treatment protocol of weekly intraarticular ACS injections.