Publikationsdatenbank

Evaluation of Two Protocols Using Autologous Conditioned Serum for Intra-articular Therapy of Equine Osteoarthritisd - A Pilot Study Monitoring Cytokines and Cartilage-Specific Biomarkers (2016)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Lasarzik, Juliane (WE 17)

Bondzio, Angelika (WE 3)

Rettig, Matthias (WE 17)

Estrada, Roberto (WE 17)

Klaus, Christoph (WE 17)

Ehrle, Anna

Einspanier, Ralf (WE 3)

Lischer, Christoph J (WE 17)

Quelle

Journal of Equine Veterinary Science — S. 1–8.e2

ISSN: 0737-0806

Sprache

Englisch

Verweise

URL (Volltext): http://dx.doi.org/10.1016/j.jevs.2016.09.014

DOI: 10.1016/j.jevs.2016.09.014

Kontakt

Institut für Veterinär-Biochemie

Oertzenweg 19 b
14163 Berlin
Tel.+49 30 838 62225 Fax.+49 30 838-62584
email:biochemie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

We hypothesised that shorter treatment intervals of intraarticular autologous conditioned serum (ACS) injections would more beneficially affect the synovial fluid (SF) concentrations of IL-1ra, IL-1β and cartilage biomarkers, compared with the traditional weekly treatment intervals in joints suffering from natural OA. In a randomised comparative study, 12 horses with OA were allocated to two groups (n = 6). The horses in group 1 received three intraarticular ACS injections at weekly intervals, whereas the horses in group 2 received three intraarticular ACS injections at two-day intervals. The levels of IL-1ra, IL-1β, CPII, C12C and CS 846 were determined in SF before and after ACS treatment using commercially available ELISA kits. The SF IL-1ra concentration 1 hour and 4 hours after ACS injection was significantly increased compared to baseline levels and decreased back to it within 48 hours. Comparing the SF IL-1ra, IL-1β, C12C, CS 846 and CP II levels before and 42 days after ACS treatment, group 2 showed a significant decrease in all parameters and an approximation on the levels in normal joints. These results indicate that the long-time effect of an ACS treatment given at two-day intervals is characterized by decreased SF IL1ra, IL-1β, C12C, CP II and CS 846 concentrations, which might indicate an improvement in joint inflammation and cartilage degrading processes . Further investigations with greater sample sizes have to prove if the two-day treatment interval is preferable to the widely used treatment protocol of weekly intraarticular ACS injections.