Publikationsdatenbank

Comprehensive resistome analysis reveals the prevalence of NDM and MCR-1 in Chinese poultry production (2017)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Wang, Yang

Zhang, Rongmin

Li, Jiyun

Wu, Zuowei

Yin, Wenjuan

Schwarz, Stefan (WE 7)

Tyrrell, Jonathan M

Zheng, Yongjun

Wang, Shaolin

Shen, Zhangqi

Liu, Zhihai

Liu, Jianye

Lei, Lei

Li, Mei

Zhang, Qidi

Wu, Congming

Zhang, Qijing

Wu, Yongning

Walsh, Timothy R

Shen, Jianzhong

Quelle

Nature microbiology

Bandzählung: 2

Heftzählung: Artikel-Nr. 16260

Seiten: 1

ISSN: 2058-5276

Sprache

Englisch

Verweise

DOI: 10.1038/nmicrobiol.2016.260

Pubmed: 28165472

Kontakt

Institut für Mikrobiologie und Tierseuchen

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 51843 / 66949
mikrobiologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

By 2030, the global population will be 8.5 billion, placing pressure on international poultry production, of which China is a key producer(1). From April 2017, China will implement the withdrawal of colistin as a growth promoter, removing over 8,000 tonnes per year from the Chinese farming sector(2). To understand the impact of banning colistin and the epidemiology of multi-drug-resistant (MDR) Escherichia coli (using blaNDM and mcr-1 as marker genes), we sampled poultry, dogs, sewage, wild birds and flies. Here, we show that mcr-1, but not blaNDM, is prevalent in hatcheries, but blaNDM quickly contaminates flocks through dogs, flies and wild birds. We also screened samples directly for resistance genes to understand the true breadth and depth of the environmental and animal resistome. Direct sample testing for blaNDM and mcr-1 in hatcheries, commercial farms, a slaughterhouse and supermarkets revealed considerably higher levels of positive samples than the blaNDM- and mcr-1-positive E. coli, indicating a substantial segment of unseen resistome-a phenomenon we have termed the 'phantom resistome'. Whole-genome sequencing identified common blaNDM-positive E. coli shared among farms, flies, dogs and farmers, providing direct evidence of carbapenem-resistant E. coli transmission and environmental contamination.