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    Molecular typing of isolates obtained from aborted foetuses in Brucella-free Holstein dairy cattle herd after immunisation with Brucella abortus RB51 vaccine in Egypt (2016)

    Art
    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Autoren
    Wareth, Gamal
    Melzer, Falk
    Böttcher, Denny
    El-Diasty, Mohamed
    El-Beskawy, Mohamed
    Rasheed, Nesma
    Schmoock, Gernot
    Roesler, Uwe (WE 10)
    Sprague, Lisa D
    Neubauer, Heinrich
    Forschungsprojekt
    Anpassung der Proteinexpression verschiedener zoonotischer Brucella Spezies an wirtsähnliche Stressbedingungen
    Quelle
    Acta tropica; 164 — S. 267–271
    ISSN: 0001-706x
    Sprache
    Englisch
    Verweise
    DOI: 10.1016/j.actatropica.2016.09.019
    Pubmed: 27664334
    Kontakt
    Institut für Tier- und Umwelthygiene

    Robert-von-Ostertag-Str. 7-13
    Gebäude 35
    14169 Berlin
    Tel.+49 30 8385 1845 Fax.+49 30 83845 1863
    email:tierhygiene@vetmed.fu-berlin.de

    Abstract / Zusammenfassung

    Bovine brucellosis is endemic in Egypt in spite of application of surveillance and control measures. An increase of abortions was reported in a Holstein dairy cattle herd with 600 animals in Damietta governorate in Egypt after immunisation with Brucella (B.) abortus RB51 vaccine. Twenty one (10.6%) of 197 vaccinated cows aborted after 3 months. All aborted cows had been tested seronegative for brucellosis in the past 3 years. B. abortus was isolated from four foetuses. Conventional biochemical and bacteriological identification and polymerase chain reaction (PCR) confirmed two B. abortus biovar (bv.) 1 smooth and two B. abortus rough strains. None of the B. abortus isolates were identified as RB51. Genotyping analysis by multiple locus of variable number tandem repeats analysis based on 16 markers (MLVA-16) revealed two different profiles with low genetic diversity. B. abortus bv1 was introduced in the herd and caused abortions.