Fachbereich Veterinärmedizin



    Characterization of the phenotypic antimicrobial resistance and biofilm profile of mastitis-associated bacteria (2016)

    Assmann, Julia (WE 7)
    Schaudinn, C.
    Janßen, T. (WE 7)
    Fidelak, C.
    Bethe, Astrid (WE 7)
    Wieler, Lothar (WE 7)
    National Symposium on Zoonoses Research 2016
    Berlin, 13. – 14.10.2016
    National Symposium on Zoonoses Research 2016 : 13 – 14 October | Berlin 2016 ; Program and Abstracts — German Research Platform for Zoonoses (Hrsg.)
    Berlin, 2016 — S. 39
    URL (Volltext): http://www.zoonosen.net/Desktopmodules/Bring2Mind/DMX/Download.aspx?EntryId=29016&PortalId=24
    Institut für Mikrobiologie und Tierseuchen

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    Gebäude 35
    14163 Berlin
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    Abstract / Zusammenfassung

    Background and objectives:
    Mastitis is a worldwide cause of enormous financial losses in dairy industry. The general therapeutical approach is the use of antibiotics, which is partly ineffective if the inflammation is caused by a biofilm forming pathogen.
    Materials and methods:
    Seven mastitis-relevant bacterial pathogens were defined and 50 isolates per bacteria MALDI-TOF MS and MICs were determined using MICRONAUT-S Mastitis 3 plates (MERLIN diagnostics). Per species, a subset of 8 isolates was chosen for biofilm experiment.
    To date, this study includes 438 isolates of clinical changed mammary gland of cows. MIC testing revealed a rather low variability between the strains and a low rate of multidrug resistant (MDR) bacteria. We were able to identify one MDR E. coli and one Methicillin-resistant S. aureus (MRSA). Most of the isolates were identified as good biofilm formers (CFU 1,0x105-1,0x107/5 mm2) compared to an EAEC known to be an excellent biofilm former (CFU 1,0x106/5 mm2). Lower CFU were determined within the tested streptococci (CFU 0-1,0x105/5 mm2).
    The heterogenic species collection showed quite sensitive resistance patterns consistent with the literature and routine diagnostic. The majority of the mastitis associated isolates were able to form a stable biofilm, which has to be considered for a successful treatment and prophylactic approaches.