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Background and objectives:
Mastitis is a worldwide cause of enormous financial losses in dairy industry. The general therapeutical approach is the use of antibiotics, which is partly ineffective if the inflammation is caused by a biofilm forming pathogen.
Materials and methods:
Seven mastitis-relevant bacterial pathogens were defined and 50 isolates per bacteria MALDI-TOF MS and MICs were determined using MICRONAUT-S Mastitis 3 plates (MERLIN diagnostics). Per species, a subset of 8 isolates was chosen for biofilm experiment.
To date, this study includes 438 isolates of clinical changed mammary gland of cows. MIC testing revealed a rather low variability between the strains and
a low rate of multidrug resistant (MDR) bacteria. We were able to identify one MDR E. coli and one Methicillin-resistant S. aureus (MRSA). Most of the isolates were identified as good biofilm formers (CFU 1,0x105-1,0x107/5 mm2) compared to an EAEC known to be an excellent biofilm former (CFU 1,0x106/5 mm2). Lower CFU were determined within the tested streptococci (CFU 0-1,0x105/5 mm2).
The heterogenic species collection showed quite sensitive resistance patterns consistent with the literature and routine diagnostic. The majority of the mastitis associated isolates were able to form a stable biofilm, which has to be considered for a successful treatment and prophylactic approaches.