Fachbereich Veterinärmedizin



    Characterization of the Phenotypic Antimicrobial Resistance and Biofilm Profile of Mastitis-Associated Bacteria (2016)

    Assmann, Julia (WE 7)
    National Symposium on Zoonoses Research 2016
    Berlin, 13. – 14.10.2016
    National Symposium on Zoonoses Research 2016 : 13 – 14 October | Berlin 2016 ; Program and Abstracts — German Research Platform for Zoonoses (Hrsg.)
    — S. 39
    URL (Volltext): http://www.zoonosen.net/Desktopmodules/Bring2Mind/DMX/Download.aspx?EntryId=29016&PortalId=24
    Institut für Mikrobiologie und Tierseuchen

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    Abstract / Zusammenfassung

    Background and objectives:
    Mastitis is a worldwide cause of enormous financial losses in dairy industry. The general therapeutical approach is the use of antibiotics, which is partly ineffective if the inflammation is caused by a biofilm forming pathogen.
    Materials and methods:
    Seven mastitis-relevant bacterial pathogens were defined and 50 isolates per bacteria MALDI-TOF MS and MICs were determined using MICRONAUT-S Mastitis 3 plates (MERLIN diagnostics). Per species, a subset of 8 isolates was chosen for biofilm experiment.
    To date, this study includes 438 isolates of clinical changed mammary gland of cows. MIC testing revealed a rather low variability between the strains and
    a low rate of multidrug resistant (MDR) bacteria. We were able to identify one MDR E. coli and one Methicillin-resistant S. aureus (MRSA). Most of the isolates were identified as good biofilm formers (CFU 1,0x105-1,0x107/5 mm2) compared to an EAEC known to be an excellent biofilm former (CFU 1,0x106/5 mm2). Lower CFU were determined within the tested streptococci (CFU 0-1,0x105/5 mm2).
    The heterogenic species collection showed quite sensitive resistance patterns consistent with the literature and routine diagnostic. The majority of the mastitis associated isolates were able to form a stable biofilm, which has to be considered for a successful treatment and prophylactic approaches.