Publikationsdatenbank

Characterization of the Phenotypic Antimicrobial Resistance and Biofilm Profile of Mastitis-Associated Bacteria (2016)

Art

Vortrag

Autor

Assmann, Julia (WE 7)

Kongress

National Symposium on Zoonoses Research 2016

Berlin, 13. – 14.10.2016

Quelle

National Symposium on Zoonoses Research 2016 : 13 – 14 October | Berlin 2016 ; Program and Abstracts — German Research Platform for Zoonoses (Hrsg.)

— S. 39

Sprache

Englisch

Verweise

URL (Volltext): http://www.zoonosen.net/Desktopmodules/Bring2Mind/DMX/Download.aspx?EntryId=29016&PortalId=24

Kontakt

Institut für Mikrobiologie und Tierseuchen

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 51843 / 66949
mikrobiologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Background and objectives:
Mastitis is a worldwide cause of enormous financial losses in dairy industry. The general therapeutical approach is the use of antibiotics, which is partly ineffective if the inflammation is caused by a biofilm forming pathogen.
Materials and methods:
Seven mastitis-relevant bacterial pathogens were defined and 50 isolates per bacteria MALDI-TOF MS and MICs were determined using MICRONAUT-S Mastitis 3 plates (MERLIN diagnostics). Per species, a subset of 8 isolates was chosen for biofilm experiment.
Results:
To date, this study includes 438 isolates of clinical changed mammary gland of cows. MIC testing revealed a rather low variability between the strains and
a low rate of multidrug resistant (MDR) bacteria. We were able to identify one MDR E. coli and one Methicillin-resistant S. aureus (MRSA). Most of the isolates were identified as good biofilm formers (CFU 1,0x105-1,0x107/5 mm2) compared to an EAEC known to be an excellent biofilm former (CFU 1,0x106/5 mm2). Lower CFU were determined within the tested streptococci (CFU 0-1,0x105/5 mm2).
Conclusion:
The heterogenic species collection showed quite sensitive resistance patterns consistent with the literature and routine diagnostic. The majority of the mastitis associated isolates were able to form a stable biofilm, which has to be considered for a successful treatment and prophylactic approaches.