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    Molecular epidemiology and genome dynamics of New Delhi metallo-beta-lactamase (NDM) producing extraintestinal pathogenic E. coli (ExPEC) strains from India (2016)

    Art
    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Autoren
    Ranjan, Amit
    Shaik, Sabiha
    Mondal, Agnismita
    Nandanwar, Nishant
    Hussain, Arif
    Semmler, Torsten
    Kumar, Narender
    Tiwari, Sumeet
    Jadhav, Savita
    Wieler, LH (WE 7)
    Ahmed, Niyaz
    Quelle
    Antimicrobial agents and chemotherapy; 60(11) — S. 6795–6805
    ISSN: 1098-6596
    Sprache
    Englisch
    Verweise
    URL (Volltext): http://aac.asm.org/content/60/11/6795
    DOI: 10.1128/AAC.01345-16
    Pubmed: 27600040
    Kontakt
    Institut für Mikrobiologie und Tierseuchen

    Robert-von-Ostertag-Str. 7-13
    Gebäude 35
    14163 Berlin
    +49 30 838 51840 / 51843
    mikrobiologie@vetmed.fu-berlin.de

    Abstract / Zusammenfassung

    The global dissemination amidst increasing incidence of carbapenem-resistant, Gram-negative organisms has resulted in acute public health concerns. Here, we present a retrospective multicenter study on molecular characterization of metallo-beta-lactamase (MBL) producing clinical E. coli isolates recovered from extraintestinal infections in two hospitals in Pune, India. We screened a large sample size of 510 E. coli isolates for MBL production wherein we profiled their molecular determinants, antimicrobial resistance phenotypes, functional virulence properties, genomic features and transmission dynamics. Approximately ∼8% of these isolates were MBL producers, the majority of which were NDM-1 (69%) type followed subsequently by NDM-5 (19%), NDM-4 (5.5%) and NDM-7 (5.5%). MBL producers were resistant to all antibiotics tested, except for colistin, fosfomycin and chloramphenicol which were effective up to a varying extent. Plasmids were found to be an effective means of dissemination of NDM genes and other resistance traits. All MBL producers adhered to and invaded bladder epithelial (T24) cells and demonstrated significant serum resistance. Genomic analysis of MBL producing E. coli revealed higher resistance but a moderate virulence gene repertoire. A subset of NDM-1 positive E. coli was identified as dominant sequence type 101 while two strains belonging to ST167 and ST405 harbored NDM-5. A majority of MBL producing E. coli strains revealed unique genotypes suggesting they were clonally unrelated. Overall, the co-existence of virulence and carbapenem resistance in clinical E. coli isolates is of serious concern. Moreover, the emergence of NDM-1 among the globally dominant E. coli ST101 warrants stringent surveillance and control measures.