Publikationsdatenbank

Combined Analysis of Variation in Core, Accessory and Regulatory Genome Regions Provides a Super-Resolution View into the Evolution of Bacterial Populations (2016)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

McNally, Alan

Oren, Yaara

Kelly, Darren

Pascoe, Ben

Dunn, Steven

Sreecharan, Tristan

Vehkala, Minna

Välimäki, Niko

Prentice, Michael B

Ashour, Amgad

Avram, Oren

Pupko, Tal

Dobrindt, Ulrich

Literak, Ivan

Guenther, Sebastian (WE 10)

Schaufler, Katharina (WE 7)

Wieler, Lothar H (WE 7)

Zhiyong, Zong

Sheppard, Samuel K

McInerney, James O

Corander, Jukka

Quelle

PLoS Genetics; 12(9) — S. 1–16

ISSN: 1553-7390

Sprache

Englisch

Verweise

DOI: 10.1371/journal.pgen.1006280

Pubmed: 27618184

Kontakt

Institut für Mikrobiologie und Tierseuchen

Robert-von-Ostertag-Str. 7-13
Gebäude 35
14163 Berlin
+49 30 838 51840 / 51843
mikrobiologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

The use of whole-genome phylogenetic analysis has revolutionized our understanding of the evolution and spread of many important bacterial pathogens due to the high resolution view it provides. However, the majority of such analyses do not consider the potential role of accessory genes when inferring evolutionary trajectories. Moreover, the recently discovered importance of the switching of gene regulatory elements suggests that an exhaustive analysis, combining information from core and accessory genes with regulatory elements could provide unparalleled detail of the evolution of a bacterial population. Here we demonstrate this principle by applying it to a worldwide multi-host sample of the important pathogenic E. coli lineage ST131. Our approach reveals the existence of multiple circulating subtypes of the major drug-resistant clade of ST131 and provides the first ever population level evidence of core genome substitutions in gene regulatory regions associated with the acquisition and maintenance of different accessory genome elements.