Fachbereich Veterinärmedizin



    Combined Analysis of Variation in Core, Accessory and Regulatory Genome Regions Provides a Super-Resolution View into the Evolution of Bacterial Populations (2016)

    Zeitschriftenartikel / wissenschaftlicher Beitrag
    McNally, Alan
    Oren, Yaara
    Kelly, Darren
    Pascoe, Ben
    Dunn, Steven
    Sreecharan, Tristan
    Vehkala, Minna
    Välimäki, Niko
    Prentice, Michael B
    Ashour, Amgad
    Avram, Oren
    Pupko, Tal
    Dobrindt, Ulrich
    Literak, Ivan
    Guenther, Sebastian (WE 10)
    Schaufler, Katharina (WE 7)
    Wieler, Lothar H (WE 7)
    Zhiyong, Zong
    Sheppard, Samuel K
    McInerney, James O
    Corander, Jukka
    PLoS Genetics; 12(9) — S. 1–16
    ISSN: 1553-7390
    DOI: 10.1371/journal.pgen.1006280
    Pubmed: 27618184
    Institut für Mikrobiologie und Tierseuchen

    Robert-von-Ostertag-Str. 7-13
    Gebäude 35
    14163 Berlin
    +49 30 838 51840 / 51843

    Abstract / Zusammenfassung

    The use of whole-genome phylogenetic analysis has revolutionized our understanding of the evolution and spread of many important bacterial pathogens due to the high resolution view it provides. However, the majority of such analyses do not consider the potential role of accessory genes when inferring evolutionary trajectories. Moreover, the recently discovered importance of the switching of gene regulatory elements suggests that an exhaustive analysis, combining information from core and accessory genes with regulatory elements could provide unparalleled detail of the evolution of a bacterial population. Here we demonstrate this principle by applying it to a worldwide multi-host sample of the important pathogenic E. coli lineage ST131. Our approach reveals the existence of multiple circulating subtypes of the major drug-resistant clade of ST131 and provides the first ever population level evidence of core genome substitutions in gene regulatory regions associated with the acquisition and maintenance of different accessory genome elements.