Fachbereich Veterinärmedizin



    Occurrence of bla VIM-1 containing Escherichia coli and Salmonella deriving from dust-samples isolated in German chicken-fattening farms (2016)

    Roschanski, N. (WE 10)
    Pietsch, M.
    Pfeiffer, Y.
    Fischer, J.
    Kreienbock, L.
    Rösler, U. (WE 10)
    National Symposium on Zoonoses Research 2016
    Berlin, 13. – 14.10.2016
    National Symposium on Zoonoses Research 2016 : 13 – 14 October | Berlin 2016 ; Program and Abstracts — German Research Platform for Zoonoses (Hrsg.)
    — S. 135–136
    URL (Volltext): http://www.zoonosen.net/Desktopmodules/Bring2Mind/DMX/Download.aspx?EntryId=29016&PortalId=24
    Institut für Tier- und Umwelthygiene

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    Abstract / Zusammenfassung

    Carbapenems are one of the last therapeutic options for the treatment of human infections caused by multidrug-resistant gramnegative bacteria. Until now, the occurrence of carbapenemases in livestock and livestock associated surroundings is rarely reported. In 2011 the first VIM-1 producing Salmonella Infantis was isolated from a German chicken-fattening farm. In the present study we investigated more than 500 primarily stored bacterial cultures, isolated in 45 chicken-fattening farms during the years 2011-2013. After a non-selective overnight incubation the bacteria were transferred to selective agar plates. Growing E. coli as well as Salmonella isolates were investigated for the presence of carbapenemase genes by real-time PCR. The received isolates are currently further investigated by using different phenotypic- as well as genotypic approaches and whole genome sequencing. Beside the already by Fischer et al., 2013 described Salmonella Infantis strain (R3), one additional Salmonella subspecies I (rough phenotype) as well as two E. coli isolates were isolated from the selective agar plates. The real-time PCR based screening indicated the presence of the blaVIM-1 gene in the newly detected Salmonella as well as in one of the two E. coli isolates. For the second E. coli strain no carbapenemase genes have been detected. Further analyses as well as comparing studies between the VIM-1 producing bacteria deriving from the different farms will certainly give more information about the serious problem of the occurrence of CPE within German
    livestock-farms. Even if the detection rate of carbapenem resistant E. coli and Salmonella was scarce, the topic is of utmost importance and a further spread of such bugs has to be avoided by all means.