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Background and objectives: High contaminaion rates of several foods with Extended-spectrum beta-lactamase (ESBL) and AmpC betalactamase-
producing Enterobacteriaceae have been reported recently. However, there is limited information on the presence of these microorganisms in seafood. The objectives of this study were to determine the prevalence and the quantitative load of ESBL/AmpC producing Enterobacteriaceae in retail seafood in Berlin, Germany.
Materials and methods: A total of 120 shrimp and mussel samples from supermarkets and seafood shops in Berlin were screened from December 2015 to April 2016 for ESBL/AmpC producing Enterobacteriaceae. ESBL/AmpC production was tested by disc difusion method and ESBL/AmpC genes were dentified using multiplex real time PCR. Direct plating on MacConkey agar supplemented with 1mg/L cefotaxime was used for enumeration of ESBL/AmpC producing Enterobacteriaceae.
Results: Overall, ESBL/AmpC producing Enterobacteriaceae were detected in 21.7% of the seafood samples with a prevalence of 25% and 18.3% for shrimp and mussels, respectively. Of the samples, 97.5% had the ESBL/AmpC producing Enterobacteriaceae counts < 100 CFU/g whereas 2.5% had counts of 100 to 1000 CFU/g. Of the 58 isolates, the most predominant ESBL/AmpC types were AmpC-CIT (20.7%), followed by SHV (12.1%) and CTX-M (8.6%).
Conclusion: Results indicate high prevalences of ESBL/AmpC producing Enterobacteriaceae in retail seafood, though the quantitative contamination level is low.