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    Prevalence of extended spectrum and AmpC β-lactamases producing Enterobacteriaceae in poultry during slaughter (2016)

    Art
    Vortrag
    Autoren
    von Tippelskirch, P. (WE 8)
    Gölz, G. (WE 8)
    Orquera, S. (WE 8)
    Projahn, M. (WE 10)
    Dähre, K. (WE 10)
    Friese, A. (WE 10)
    Rösler, U. (WE 10)
    Alter, T. (WE 8)
    Kongress
    National Symposium on Zoonoses Research 2016
    Berlin, 13. – 14.10.2016
    Quelle
    National Symposium on Zoonoses Research 2016 : 13 – 14 October | Berlin 2016 ; Program and Abstracts — German Research Platform for Zoonoses (Hrsg.)
    — S. 62
    Sprache
    Englisch
    Verweise
    URL (Volltext): http://www.zoonosen.net/Desktopmodules/Bring2Mind/DMX/Download.aspx?EntryId=29016&PortalId=24
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    Institut für Lebensmittelsicherheit und -hygiene

    Königsweg 69
    14163 Berlin
    Tel.+49 30 838 62550 Fax.+49 30 838 46029
    email:lebensmittelhygiene@vetmed.fu-berlin.de

    Abstract / Zusammenfassung

    Background and objectives: Extended spectrum and AmpC ßlactamase forming Enterobacteriaceae hydrolyse ß-lactam antibiotics, such as penicillines and cephalosporines. As food producing animals are considered a reservoir for these bacteria, meat represents a possible source of infection for humans. Other studies already showed high prevalence of ESBL/AmpC forming Enterobacteriaceae in poultry, however not much is known about the exact number of ESBL/AmpC-forming Enterobacteriaceae during slaughter.
    Materials and methods: Samples of four chicken flocks were screened qualitatively and quantitatively for ESBL/AmpC producing Enterobacteriaceae using MacConkey agar containing cefotaxime. Species were identified by MALDI-TOF and antimicrobial susceptibility tested by disk diffusion assays. The presence of ESBL/AmpC genes (blaTEM, blaSHV, blaCTX, blaCMY) was determined by RT-PCR in all cefotaxime resistant Enterobacteriaceae isolates. Results: On average, 40 % (40/100) of caecal, 75 % (75/100) of skin, 23 % (23/100) of meat and 35 % (18/52) of environmental samples contained ESBL/AmpC forming Enterobacteriaceae. The median number of the cefotaxime resistant Enterobacteriaceae was higher in caecum and skin (1 x 103 CFU/g) than in meat (7 x 101 CFU/g).
    Conclusion: Although prevalence was high during poultry slaughter, total number of ESBL/AmpC forming Enterobacteriaceae was lower than expected.