Fachbereich Veterinärmedizin



    Impact of dextran sulphate sodium-induced colitis on the intestinal transport of the colon carcinogen PhIP (2016)

    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Nicken, Petra
    von Keutz, Anne
    Willenberg, Ina
    Ostermann, Annika I
    Schebb, Nils Helge
    Giovannini, Samoa (WE 12)
    Breves, Gerhard
    Steinberg, Pablo
    Kershaw, Olivia (WE 12)
    Archives of toxicology; 90(5) — S. 1093–1102
    ISSN: 0340-5761
    DOI: 10.1007/s00204-015-1546-1
    Pubmed: 26070365
    Institut für Tierpathologie

    Robert-von-Ostertag-Str. 15
    Gebäude 12
    14163 Berlin
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    Abstract / Zusammenfassung

    Colorectal cancer is one of the most frequent cancers in Western countries. Chronic intestinal diseases such as Crohn's disease and ulcerative colitis, in which the intestinal barrier is massively disturbed, significantly raise the risk of developing a colorectal tumour. 2-Amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) is a genotoxic heterocyclic aromatic amine that is formed after strongly heating fish and meat. In this study, the hypothesis that PhIP uptake in the gut is increased during chronic colitis was tested. Chronic colitis was induced by oral administration of dextran sulphate sodium (DSS) to Fischer 344 rats. The transport of PhIP in eight different rat intestinal segments was examined in Ussing chambers. The tissues were incubated with 10 µM PhIP for 90 min, and the concentration of PhIP was determined in the mucosal and serosal compartments of the Ussing chambers as well as in the clamped tissues by LC-MS. Although chronic colitis was clearly induced in the rats, no differences in the intestinal transport of PhIP were observed between control and DSS-treated animals. The hypothesis that in the course of chronic colitis more PhIP is taken up by the intestinal epithelium, thereby increasing the risk of developing colorectal cancer, could not be confirmed in the present report.