Publication Database

Genetic diversity of Clostridium perfringens isolates from healthy and deseased poultry in Egypt and Germany (2015)

Art

Poster

Autoren

Abdel-Glil, M.

Semmler, Torsten

Eichhorn, Inga (WE 7)

Lübke-Becker, Antina (WE 7)

Wieler, L. H.

Neubauer, H.

Seyboldt, C. (WE 7)

Kongress

Junior Scientis Symposium 2015

FLI, 21. – 23.09.2015

Quelle

Junior Scientis Symposium 2015 : Abstract collection — Friedrich-Loeffler-Institut, Bundesforschungsinstitut für Tiergesundheit (Hrsg.)

— S. 12

Sprache

Englisch

Verweise

URL (Volltext): https://openagrar.bmel-forschung.de/servlets/MCRFileNodeServlet/Document_derivate_00013099/Abstractband-NWS_3.pdf;jsessionid=9065AAAD0890F3A028EB5E7906A46D21

Kontakt

Institut für Mikrobiologie und Tierseuchen

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 51843 / 66949
mikrobiologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Necrotic enteritis (NE) is an enteric disease of poultry caused mainly by Clostridium (C.) perfringens type A. The disease re-emerged due to increasing restrictions of routine use of antimicrobial growth promoters in poultry farming in Europe and worldwide. The pathogenesis of NE seems to be multifactorial and de-pends on bacterial virulence factors, host-related factors such as stress, and predisposing factors e.g. coc-cidiosis. C. perfringens is supposed to use an arsenal of more than 16 toxins to cause intestinal infections in animals and humans. C. perfringens alpha toxin (CPA) has been considered a major virulence factor for NE but its role is still not clear. Additionally a novel so called Necrotic Enteritis Toxin B-like (NetB toxin) is believed to play a key role in NE pathogenesis. However, the detection of netB-negative strains associated with NE in poultry and netB-positive strains in healthy birds has called its impact into question. Our aim is to determine the prevalence of known virulence elements within C. perfringens strains isolated from healthy and infected chickens from different regions. In addition we use Illumina/Solexa sequencing tech-nology to generate whole genome sequence data for selected strains in order to identify NE strain-specific markers.