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    Microevolution of S. pseudinermedius isolated from Infections of one canine patien at 25 different ime points between 2008 and 2014 (2015)

    Art
    Vortrag
    Autoren
    Vincze, Szilvia (WE 7)
    Walther, Birgit (WE 7)
    Wieler, LH
    Kohn, B (WE 20)
    Brunnberg, Leo (WE 20)
    Luebke-Becker, Antina (WE 7)
    Kongress
    4th ASM-ESCMID Conference on Methicillin-resistant Staphylococci in Animals: Veterinary and Public Health Implications
    Chicago, Illinois, 02. – 05.11.2015
    Quelle
    4th ASM-ESCMID Conference on Methicillin-resistant Staphylococci in Animals: Veterinary and Public Health Implications ; November 2 – 5, 2015 Chicago, Illinois ;Final Program and Abstracts — American Society for Microbiology (Hrsg.)
    Washington, DC, 2015 — S. 24
    Sprache
    Englisch
    Verweise
    URL (Volltext): http://conferences.asm.org/images/mrsa2015finalprogram.pdf
    Kontakt
    Institut für Mikrobiologie und Tierseuchen

    Robert-von-Ostertag-Str. 7-13
    Gebäude 35
    14163 Berlin
    +49 30 838 51840 / 51843
    mikrobiologie@vetmed.fu-berlin.de

    Abstract / Zusammenfassung

    Introduction: The opportunistic pathogen S. pseudintermedius mainly causes purulent infections in dogs. Recurrent infections have been described in the past. In order to unravel the microevolution as well as the phenotypic diversity of this pathogen within one patient phenotypic and genotypic characterization was performed for methicillin-usceptible (MSSP) and methicillin-resistant S. pseudintermedius (MRSP) isolated from multiple wound infections of a dog during a seven-year time period. Material and Methods: Between 2008 and
    2014, S. pseudintermedius was isolated from 25 wound swabs from one patient. In total, 38 isolates (up to eight colonies / swab) were sequenced. Clonal relationship was determined based on the allele diversity of 1845 target genes (Ridom SeqSphere + 2.3.1). Variability within isolates of distinct genotypes (gene content and SNPs [single nucleotide olymorphisms]) was analyzed (geneious 6.1.5). To display the phylogenetic relationship for isolates of each lineage neighbor-joining trees were built. The binding capacity to ibrinogen and fibronectin as well as biofilm formation was determined for eleven isolates. Results: MLST+ typing revealed three distinct genotypes (I, II, III). All MRSP (n=21) elonged to genotype I. MSSP clustered into two genotypes II (n=13) and III (n=2). Lineages I and II contained the majority of isolates. Within each of these two predominant lineages only minor variations were detected regarding the gene content and SNPs. Despite the low number of identified SNPs (MRSP-I n=27; MSSP-II n=17), an accumulation was observed over the time for both lineages. While MRSP-I showed only weak adherence to fibrinogen, MSSP-III
    had a moderate and MSSP-II a strong binding capacity. Strong biofilm formation was observed for all MRSP. Isolates sharing the same genetic background displayed a comparable
    phenotypic profile. Discussion: Sampling of 25 wound infections from one patient revealed two different successful genetic lineages. Interestingly, isolates sharing the same genetic
    background showed only minor genetic variation even though the strains were isolated over seven years. While mixed infections with MRSP-I and MSSP-II were determined twice, exchange of mobile genetic elements was not detected. Phenotyping revealed opposing abilities for MRSP-I and MSSP-II regarding adherence to fibrinogen and biofilm formation, indicating that none of these tested mechanisms is essential for S. pseudintermedius to successfully infect dogs. The lack of phenotypic variability of isolates sharing the same genetic background is in accordance with the stable genome of these strains. A reasonable explanation for the lack of variability within the identified lineages might be recurrent autoinfections or a persistent infection rather than re-infections due to an external ource.