Fachbereich Veterinärmedizin



    Know your neighbourhood:
    Antibiotic resistance profiling of staphylococcal species from livestock environments neighbouring LA-MRSA (2015)

    Ziebuhr, W.
    Schoenfelder, S.
    Ying, D.
    Walther, B. (WE 7)
    Köck, R.
    67. Jahrestagung der Deutschen Gesellschaft für Hygiene und Mikrobiologie (DGHM)
    Münster, 27. – 30.09.2015
    International journal of medical microbiology; 305(Supplement 1) — S. 118
    ISSN: 1438-4221
    URL (Volltext): http://linkinghub.elsevier.com/retrieve/pii/S1438422115001009
    DOI: 10.1016/j.ijmm.2015.09.002
    Institut für Mikrobiologie und Tierseuchen

    Robert-von-Ostertag-Str. 7-13
    Gebäude 35
    14163 Berlin
    +49 30 838 51840 / 51843

    Abstract / Zusammenfassung

    In this study we focused on the antibiotic resistance profiles of livestock-associated coagulase-negative staphylococci (LA-CoNS) to assess their risk potential for horizontal gene transfer of known or novel antibiotic resistance genes into LA-MRSA. Among 400 LA-CoNS isolates from dust and manure samples in diverse animal husbandries, we identified 20 different staphylococcal species, with S. sciuri being the most abundant species (45%), followed by S. simulans (14%), S. chromogenes (10%), S. pasteuri (7%) and S. haemolyticus (6%). High resistance rates were detected for tetracycline (70%), oxacillin and penicillin (65%), fusidic acid (52%), clindamycin (55%) and erythromycin (40%), while for other antibiotics, e.g. rifampicin, vancomycin, gentamicin and quinupristin-dalfopristin, LA-CoNS showed high susceptibility. Moreover, intrinsic resistance to fusidic acid and a lower susceptibility to moxifloxacin were found among S. sciuri, which dominated the population in the livestock environment. We also tested the MIC levels of daptomycin, a last resort antibiotic in the treatment of MRSA infections, and detected an alarmingly high
    tolerance to daptomycin (average MIC levels of 4 μg/ml) among LA-CoNS and S. sciuri. In particular, two S. sciuri isolates were demonstrated to exhibit high-level daptomycin resistance. Future work aims at the identification of the molecular mechanism behind the daptomycin resistance phenotype to weigh the danger of horizontal gene transfer to MRSA/VRSA.