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    Positive predictive value of isolation of potentially ESBL-producing Escherichia coli on MacConkey agar containing 1 mg/l cefotaxime (2015)

    Art
    Vortrag
    Autoren
    Hille, K. (WE 2)
    Hering, J.
    Friese, A. (WE 10)
    Fischer, A.
    Guerra, B.
    Rösler, U. (WE 10)
    Kreienbrock, L.
    Kongress
    National Symposium on Zoonoses Research
    Berlin, 15.10.2015 – 16.10.2016
    Quelle
    National Symposium on Zoonoses Research : 15 - 16 October 2015 ; Program and Abstracts — German Research Platform for Zoonoses (Hrsg.)
    Berlin, 2015 — S. 122
    Sprache
    Englisch
    Kontakt
    Institut für Tier- und Umwelthygiene

    Robert-von-Ostertag-Str. 7-13
    Gebäude 35
    14169 Berlin
    +49 30 838 51845
    tierhygiene@vetmed.fu-berlin.de

    Abstract / Zusammenfassung

    Extended spectrum beta-lactamases (ESBL) belong to the most widely disseminated resistance mechanisms in Enterobacteriacae. According to a common isolation protocol, also recommended by the European Food Safety Authority (EFSA), the isolation starts with an enrichment culture followed by cultivation on MacConkey agar plates containing 1 mg/l cefotaxime. ESBL-production is then confirmed by antimicrobial susceptibility testing with specific panels and PCR of the ESBL-genes.
    We were interested in the positive predictive value (PPV) of the cultural isolation on MacConkey agar plates containing 1 mg/l cefotaxime for ESBL-production. Therefore ESBL-producing E. coli samples from broiler, fattening pig and cattle farms were first isolated culturally. Subsequently ESBL suspicious isolates were confirmed by antimicrobial susceptibility testing and PCR of ESBL-genes.
    From samples collected on fattening pig farms 121 isolates growing on MacConkey agar plates containing 1 mg/l cefotaxime were obtained. Of these isolates 107 could be confirmed by PCR. It follows a PPV of 87%. In the presentation, the results of PPV for the different sample sources will be reported. The calculation of the sensitivity and specificity from these data and the possible limitations of this method will be explained.