Fachbereich Veterinärmedizin


Service-Navigation

    Publikationsdatenbank

    Pathogenesis of infection with 2009 pandemic H1N1 influenza virus in isogenic guinea pigs after intranasal or intratracheal inoculation (2015)

    Art
    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Autoren
    Wiersma, Lidewij C M
    Vogelzang-van Trierum, Stella E
    van Amerongen, Geert
    van Run, Peter
    Nieuwkoop, Nella J
    Ladwig, Mechtild (WE 11)
    Banneke, Stefanie
    Schaefer, Hubert
    Kuiken, Thijs
    Fouchier, Ron A M
    Osterhaus, Albert D M E
    Rimmelzwaan, Guus F
    Quelle
    American journal of pathology; 185(3) — S. 643–650
    ISSN: 0002-9440
    Sprache
    Englisch
    Verweise
    DOI: 10.1016/j.ajpath.2014.11.012
    Pubmed: 25555619
    Kontakt
    Institut für Tierschutz und Tierverhalten

    Königsweg 67
    Gebäude 21, 1. OG
    14163 Berlin
    +49 30 838 62901
    tierschutz@vetmed.fu-berlin.de

    Abstract / Zusammenfassung

    To elucidate the pathogenesis and transmission of influenza virus, the ferret model is typically used. To investigate protective immune responses, the use of inbred mouse strains has proven invaluable. Here, we describe a study with isogenic guinea pigs, which would uniquely combine the advantages of the mouse and ferret models for influenza virus infection. Strain 2 isogenic guinea pigs were inoculated with H1N1pdm09 influenza virus A/Netherlands/602/09 by the intranasal or intratracheal route. Viral replication kinetics were assessed by determining virus titers in nasal swabs and respiratory tissues, which were also used to assess histopathologic changes and the number of infected cells. In all guinea pigs, virus titers peaked in nasal secretions at day 2 after inoculation. Intranasal inoculation resulted in higher virus excretion via the nose and higher virus titers in the nasal turbinates than intratracheal inoculation. After intranasal inoculation, infectious virus was recovered only from nasal epithelium; after intratracheal inoculation, it was recovered also from trachea, lung, and cerebrum. Histopathologic changes corresponded with virus antigen distribution, being largely limited to nasal epithelium for intranasally infected guinea pigs and more widespread in the respiratory tract for intratracheally infected guinea pigs. In summary, isogenic guinea pigs show promise as a model to investigate the role of humoral and cell-mediated immunities to influenza and their effect on virus transmission.