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This preliminary study has conducted to detect the presence of Extended Spectrum Beta Lactamase producing Escherichia coli (ESBLEC) from commercial broiler farms in Jaffna district, Sri Lanka and to assess the risk factors regarding farm management from questionnaires. Pooled fecal and paired boot swabs samples were collected from 83 broiler farms. Both types of samples were individually processed for ESBL confirmation along with antibiotic susceptibility testing using disk diffusion methods (CLSI 2014). ESBL-EC were
detected from 50.6% of samples from broiler farms. Isolation rates were 45.95% (34/74) from boots samples and 40.96% (34/83) from fecal samples. Antimicrobial resistance of fecal ESBL-EC isolates were Ciprofloxacin (91.2%), Nalidixic Acid (91.2%), Enrofloxacin (85.3%), Trimethoprim/Sulfamethoxazole (85.3%), Tetracycline (85.3%), Doxycline (52.9%), Amoxacillin/Clavulanic acid (17.6%), Chloramphenicol (14.7%). All isolates were susceptible to Imipenem and Nitrofurantoin. Furthermore, same ESBL-EC isolates had observed resistances to 91.2% of Quinolones, 85.3% of Folate pathways inhibitors and 79.4% of Fluoroquinolones.