Fachbereich Veterinärmedizin



    Feeding of Enterococcus faecium NCIMB 10415 leads to intestinal miRNA-423-5p induced regulation of immune-relevant genes (2016)

    Zeitschriftenartikel / wissenschaftlicher Beitrag
    Kreuzer-Redmer, Susanne
    Bekurtz, Jennifer C
    Arends, Danny
    Bortfeldt, Ralf
    Kutz-Lohroff, Barbara (WE 3)
    Sharbati, Soroush (WE 3)
    Einspanier, Ralf (WE 3)
    Brockmann, Gudrun A
    Applied and environmental microbiology; 82(8) — S. 2263–2269
    ISSN: 0099-2240
    DOI: 10.1128/AEM.04044-15
    Pubmed: 26826223
    Institut für Veterinär-Biochemie

    Oertzenweg 19 b
    14163 Berlin
    +49 30 838 62225

    Abstract / Zusammenfassung

    Probiotics are widely used in human and animal health, but little is known about the mode of action of probiotics. One possible mechanism on a molecular level could be an influence on miRNAs and the related immune-relevant target genes. Here, we analyzed differential expression of miRNA and potential target genes of ileal and jejunal lymphatic tissue from Enterococcus faecium NCIMB 10415-fed piglets versus untreated controls using Next Generation Sequencing. We identified the miR-423-5p as highly affected by the treatment group (2.32 fold; P = 0.014). Validation by RT-qPCR confirmed a significant up-regulation of miR-423-5p (2.11 fold; P = 0.03) and, additionally a down-regulation of important immune-relevant target gene IGLC (0.61 fold; P = 0.03) and IGKC (0.69 fold; P = 0.04). Expression analysis of miR-423-5p and IGLC at different age points, show a clear anti-correlated relation. Luciferase reporter assays in a HeLa cell line verified IGLC as a target of miR-423-5p. The results provided evidence for an effect of feeding E. faecium on the expression of miR-423-5p and on the regulation of the immune globulin gene IGLC through miR-423-5p. This might be a possible mode of action of E. faecium on immune cell regulation in the small intestine.